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Intersectional targeting of defined neural circuits by adeno‐associated virus vectors
Journal of Neuroscience Research ( IF 4.2 ) Pub Date : 2020-12-20 , DOI: 10.1002/jnr.24774
Chase A Weinholtz 1 , Michael J Castle 2
Affiliation  

The mammalian nervous system is a complex network of interconnected cells. We review emerging techniques that use the axonal transport of adeno‐associated virus (AAV) vectors to dissect neural circuits. These intersectional approaches specifically target AAV‐mediated gene expression to discrete neuron populations based on their axonal connectivity, including: (a) neurons with one defined output, (b) neurons with one defined input, (c) neurons with one defined input and one defined output, and (d) neurons with two defined inputs or outputs. The number of labeled neurons can be directly controlled to trace axonal projections and examine cellular morphology. These approaches can precisely target the expression of fluorescent reporters, optogenetic ion channels, chemogenetic receptors, disease‐associated proteins, and other factors to defined neural circuits in mammals ranging from mice to macaques, and thereby provide a powerful new means to understand the structure and function of the nervous system.

中文翻译:

腺相关病毒载体对确定的神经回路的交叉靶向

哺乳动物的神经系统是一个由相互连接的细胞组成的复杂网络。我们回顾了使用腺相关病毒 (AAV) 载体的轴突运输来解剖神经回路的新兴技术。这些交叉方法专门针对基于轴突连接性的离散神经元群体的 AAV 介导的基因表达,包括:(a) 具有一个定义输出的神经元,(b) 具有一个定义输入的神经元,(c) 具有一个定义输入和一个定义的神经元定义的输出,以及 (d) 具有两个定义的输入或输出的神经元。可以直接控制标记神经元的数量以追踪轴突投射并检查细胞形态。这些方法可以精确靶向荧光报告基因、光遗传学离子通道、化学遗传学受体、疾病相关蛋白、
更新日期:2021-02-21
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