Zamzam water is a natural alkaline water which has become alkaline as a result of the natural environment. It comes from what is considered as one of the oldest springs in the world. The water contains high concentrations of alkaline minerals as well as trace and heavy metals. The aim of the current study is to evaluate the effects of five weeks ingestion of Zamzam water on the liver and kidney functions of rats. Adult female Wistar rats weighing 150–200 g were divided into two groups, with 15 rats in each. The control group was supplied daily by bottled water and the Zamzam water group was supplied daily by 500 ml of Zamzam water for five weeks. The rats were weighed weekly and, at the end of the experiment, blood samples were collected from all rats for the biochemical determination of serum levels of aspartate transaminase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), urea, creatinine, albumin, and uric acid, using calorimetric methods. Liver and kidney tissues were fixed in 10% neutral buffered-formalin solution and further embedded in wax blocks for routine hematoxylin and eosin (H&E) staining and were examined for histopathological changes using a light microscope. The results of the current study showed that there was a significant increase (P < 0.05) in the weight of the Zamzam group when compared to the control group after five weeks of ingestion. Liver and kidney function tests did not show any significant difference when compared with the controls (P > 0.05). In addition, histological examination of the liver and kidney tissues did not show any toxicological changes. In conclusion, the results showed that the ingestion of Zamzam water did not alter serum levels of kidney function tests and liver enzymes; and did not result in a noticeable change in the liver and kidney histology. Thus, the high concentrations of elements in Zamzam water do not induce hepatotoxicity or nephrotoxicity and the water is considered safe for long-term consumption.

扎姆扎姆水是一种天然碱性水，由于自然环境而变成碱性。它来自被认为是世界上最古老的温泉之一。水中含有高浓度的碱性矿物质以及微量和重金属。本研究的目的是评估五周摄入扎姆扎姆水对大鼠肝肾功能的影响。将体重150-200 g的成年雌性Wistar大鼠分为两组，每组15只。对照组每天用瓶装水供应，Zamzam水组每天用500毫升Zamzam水供应，持续5周。每周对大鼠称重，在实验结束时，从所有大鼠中收集血样，用于生化测定血清天冬氨酸转氨酶（AST）的水平，丙氨酸氨基转移酶（ALT），碱性磷酸酶（ALP），尿素，肌酐，白蛋白和尿酸，采用量热法。将肝和肾组织固定在10％中性福尔马林缓冲液中，然后进一步包埋在蜡块中，以进行常规苏木精和曙红（H＆E）染色，并使用光学显微镜检查其组织病理学变化。目前的研究结果显示， 摄入五周后，与对照组相比，Zamzam组的体重P <0.05）。与对照组相比，肝肾功能检查未显示任何显着差异（P  > 0.05）。另外，肝和肾组织的组织学检查未显示任何毒理学变化。总之，结果表明，摄入扎姆扎姆水不会改变血清肾功能测试和肝酶水平。并没有导致肝脏和肾脏组织学发生明显变化。因此，扎姆扎姆水中的高浓度元素不会引起肝毒性或肾毒性，因此认为该水可长期食用。