Purpose: The high expression of positive regulatory domainzinc finger protein2 (PRDM2) is an important factor in inducing the formation and progression of gastric cancer. The current study was performed to explore the effect of micro-RNA-362 (miR-362) targeting PRDM2 on the proliferation and apoptosis of gastric cancer cells. Methods: The expression of miR-362 in gastric adenocarcinoma and normal gastric mucosa was detected by real-time fluorescence quantitative PCR (qPCR), and the expression of PRDM2 in gastric adenocarcinoma was detected by immunohistochemical method. Gastric cancer cell line MGC-803 and human normal gastric mucosal epithelial cell line (GES-1) were selected for study. Blank control group, empty vector transfection group, miR-362 transfection group, and miR-362 and PRDM2 co-transfection group were established. CCK-8 assay was utilized to detect cell activity, flow cytometry was used to detect cell cycle and apoptosis, and invasion capability of cells was observed through Transwell experiment. The expression of Cyclin D1 and Caspases-3 was detected by Western Blot method. Results: The expression of miR-362 in gastric adenocarcinoma was significantly lower than that in normal gastric mucosa. The expression of miR-362 in gastric adenocarcinoma was significantly different in the maximum diameter, depth of invasion and different TNM stages. The expression of miR-362 was linked to prognosis. In addition, there was a negative correlation between miR-362 and PRDM2. The expression of miR-362 in gastric cancer cell line MGC-803 was significantly lower than that in GES-1. Compared with the blank control group and empty vector transfection group, the proliferat

中文翻译：

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miR-362通过抑制促癌因子PRDM2表达调控胃癌增殖、侵袭和凋亡的作用

目的：正调控域锌指蛋白2（PRDM2）的高表达是诱导胃癌形成和进展的重要因素。本研究旨在探讨靶向 PRDM2 的 micro-RNA-362 (miR-362) 对胃癌细胞增殖和凋亡的影响。方法:采用实时荧光定量PCR(qPCR)法检测miR-362在胃腺癌和正常胃黏膜中的表达,免疫组化法检测胃腺癌中PRDM2的表达。选择胃癌细胞系MGC-803和人正常胃粘膜上皮细胞系（GES-1）进行研究。建立空白对照组、空载体转染组、miR-362转染组、miR-362与PRDM2共转染组。采用CCK-8法检测细胞活性，流式细胞术检测细胞周期和凋亡，Transwell实验观察细胞侵袭能力。Western Blot法检测Cyclin D1和Caspases-3的表达。结果：miR-362在胃腺癌中的表达明显低于正常胃黏膜。miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖细胞 流式细胞仪检测细胞周期和凋亡，Transwell实验观察细胞侵袭能力。Western Blot法检测Cyclin D1和Caspases-3的表达。结果：miR-362在胃腺癌中的表达明显低于正常胃黏膜。miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖 流式细胞仪检测细胞周期和凋亡，Transwell实验观察细胞侵袭能力。Western Blot法检测Cyclin D1和Caspases-3的表达。结果：miR-362在胃腺癌中的表达明显低于正常胃黏膜。miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖 Transwell实验观察细胞的侵袭能力。Western Blot法检测Cyclin D1和Caspases-3的表达。结果：miR-362在胃腺癌中的表达明显低于正常胃黏膜。miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖 Transwell实验观察细胞侵袭能力。Western Blot法检测Cyclin D1和Caspases-3的表达。结果：miR-362在胃腺癌中的表达明显低于正常胃黏膜。miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖 Western Blot法检测Cyclin D1和Caspases-3的表达。结果：miR-362在胃腺癌中的表达明显低于正常胃黏膜。miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖 Western Blot法检测Cyclin D1和Caspases-3的表达。结果：miR-362在胃腺癌中的表达明显低于正常胃黏膜。miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖 miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖 miR-362在胃腺癌中的表达在最大直径、浸润深度和不同TNM分期方面存在显着差异。miR-362的表达与预后有关。此外，miR-362和PRDM2之间存在负相关。miR-362在胃癌细胞株MGC-803中的表达明显低于GES-1。与空白对照组和空载体转染组相比，增殖