Interferon Regulatory Factor-3 (IRF-3) is one of the key players in the inflammatory response mediated by the innate immune system. Although many studies have implicated a role for IRF-3 in the pathogenesis of inflammatory airway diseases, information about the possible association of IRF-3 genetic variants with asthma is scarce. We aimed to investigate the potential effects of IRF-3 polymorphisms in childhood asthma and asthma-related phenotypes. IRF-3 polymorphisms were first determined by sequencing 25 asthmatic and 25 healthy children. For further analysis, 609 asthmatic children and 191 healthy controls were screened for the genetic variants, such as rs2304204, rs2304205, rs320440, rs34739574, and rs7251. In addition, the relationship between these polymorphisms and asthma-related phenotypic features, including forced expiratory volume in one second values, eosinophil counts, and IgE levels was determined. rs7251 was associated with asthma in the codominant (P = 0.049) and G dominant (P = 0.025) model, however this significance was lost after False Discovery Rate analysis. Other investigated single nucleotide polymorphisms (SNPs) showed no significant association with asthma or asthma-related phenotypes. In conclusion, the seven SNPs of IRF-3 gene are not associated with asthma or asthma-related phenotypes in Turkish asthmatic children.

中文翻译：

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哮喘患儿干扰素调节因子 3 的遗传变异

干扰素调节因子 3 (IRF-3) 是先天免疫系统介导的炎症反应的关键参与者之一。尽管许多研究表明 IRF-3 在炎症性气道疾病的发病机制中起作用，但关于 IRF-3 遗传变异与哮喘可能关联的信息很少。我们旨在调查IRF-3多态性对儿童哮喘和哮喘相关表型的潜在影响。IRF-3多态性首先通过对 25 名哮喘儿童和 25 名健康儿童进行测序来确定。为了进一步分析，筛选了 rs2304204、rs2304205、rs320440、rs34739574 和 rs7251 等遗传变异的 609 名哮喘儿童和 191 名健康对照。此外，还确定了这些多态性与哮喘相关表型特征（包括一秒用力呼气量值、嗜酸性粒细胞计数和 IgE 水平）之间的关系。rs7251 与共显性 ( P  = 0.049) 和 G 显性 ( P = 0.025) 模型，但是在 False Discovery Rate 分析之后，这种重要性消失了。其他研究的单核苷酸多态性 (SNP) 显示与哮喘或哮喘相关表型没有显着关联。总之，IRF-3基因的七个 SNP与土耳其哮喘儿童的哮喘或哮喘相关表型无关。