Abstract

The analysis of DNA fragments by capillary gel electrophoresis is in wide current used in the practice of research and testing laboratories primarily for highly accurate and highly sensitive identification of organisms by their DNA traces separated from biological test materials and amplified by polymerase chain reaction. A constantly growing flow of examinations performed using this method necessitates the optimization of research protocols in order to shorten the analysis time without loss of separation efficiency. The effect of electric field voltage on the separation efficiency of single-stranded DNA fragments in a length range from 60 to 1200 nucleotides in a series of polymers based on linear poly(N,N-dimethylacrylamide) was studied. The best values of electric field voltage were experimentally determined to be recommended for DNA fragment analysis on the first Russian genetic analyzer Nanophore 05 with the studied polymers.

中文翻译：

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电场电压对线性聚（N，N-二甲基丙烯酰胺）聚合物中毛细管凝胶电泳分离单链DNA片段效率的影响

摘要

通过毛细管凝胶电泳对DNA片段进行分析已广泛用于研究和测试实验室的实践中，主要用于通过从生物测试材料中分离并通过聚合酶链反应扩增的DNA痕迹对生物进行高精度和高灵敏度的鉴定。使用这种方法进行的检查流程不断增长，因此有必要优化研究方案，以缩短分析时间而不损失分离效率。电场电压对基于线性聚（N，N）的一系列聚合物中长度为60至1200个核苷酸的单链DNA片段分离效率的影响-二甲基丙烯酰胺）的研究。实验确定了最佳的电场电压值，建议将其用于第一台带有研究聚合物的俄罗斯遗传分析仪Nanophore 05上的DNA片段分析。