Abstract

The chromatographic analysis of food products for trans isomers of monounsaturated fatty acids, which are harmful components, in the presence of an excess of useful cis isomers is considered as a complex problem of considerable current interest. The separation of substances by gas–liquid chromatography occurs in accordance with two mechanisms with different selectivity for geometric isomers based on adsorption on a flat surface, which gives an energy advantage to trans isomers, and distribution in the bulk phase, which leads to the stronger retention of cis isomers. The theoretical model proposed previously to explain the distribution of an adsorbate as its “absorption” by a stationary-phase macromolecule due to a conformational rearrangement in the course of nonspecific interaction was supplemented by polar interactions and hydrogen bonds. The factors influencing the selectivity of a phase for geometric isomers were considered. A theoretical analysis of the experimental data was carried out and the parameters of stationary phases required for increasing the separation selectivity of the trans and cis isomers of fatty acid esters were determined.

中文翻译：

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气相色谱分析中确定脂肪酸几何异构体固定相选择性的因素

摘要

在存在大量有用的顺式异构体的情况下，对食品中的有害成分单不饱和脂肪酸的反式异构体进行色谱分析，被认为是当前令人关注的复杂问题。气相色谱法分离物质是根据两种机理进行的，这两种机理是基于在平面上的吸附，对几何异构体具有不同的选择性，这为反式异构体提供了能量优势，并且可以在本体相中分布，从而导致更强的分离能力。顺式保留异构体。先前提出的理论模型用来解释由于非特异性相互作用过程中构象重排而导致的固定相大分子吸附物被固定相大分子“吸收”的分布，并通过极性相互作用和氢键得到补充。考虑了影响相的几何异构体选择性的因素。实验数据的理论分析进行和用于增加的分离选择性所需的固定相的参数反式和CIS测定的脂肪酸酯的异构体。