Abstract

Auxins play a very important role in plant development and growth. Herein a rapid and convenient method was developed for the quantification of total auxins including indole-3-acetic acid, indole-3-propionic acid and indole-3-butyric acid in mung bean sprouts by means of spectrofluorometry. Based on the same fluorescence properties of the compounds, a linear working curve was established between total concentrations of auxins and fluorescence intensities of their solutions. Under optimized conditions, correlation coefficient and limit of detection were found to be 0.9993 and 0.03 μg/mL, respectively, in linear range of 0.10–24 μg/mL. This method was successfully employed to the determination of total auxins after their extraction from mung bean sprouts, and the recovery was found to be in the range of 100–104% with RSD below 5%. This method has been proved to be simple, reproducible and accurate, also applicable for food samples without complicated apparatus and procedures.

中文翻译：

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一种简单的荧光分光光度法测定绿豆芽中的总生长素

摘要

生长素在植物发育和生长中起着非常重要的作用。本文开发了一种快速方便的方法，通过分光荧光法定量绿豆芽中的总生长素，包括吲哚-3-乙酸，吲哚-3-丙酸和吲哚-3-丁酸。基于化合物的相同荧光特性，在植物生长素的总浓度与溶液的荧光强度之间建立了线性工作曲线。在最佳条件下，相关系数和检出限分别在0.10–24μg/ mL的线性范围内，分别为0.9993和0.03μg/ mL。该方法已成功用于从绿豆芽中提取后的总生长素的测定，发现回收率在100–104％范围内，RSD低于5％。