Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks in vivo by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods.

中文翻译：

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注射伊文思蓝染料以对完整的脉管系统进行荧光标记和成像。

血管在健康和疾病中都发挥着关键作用。了解血管如何形成、模式和对损坏的反应至关重要。然而，标记和成像脉管系统以确定这些特性可能既困难又耗时。在这里，作者提出了一种通过利用伊文思蓝的荧光特性快速有效地标记体内整个血管网络的新方法。通过将标记与荧光显微镜相结合，与传统方法相比，这种方法能够以一小部分时间和成本实现整个组织脉管系统的可视化。