Area-wide integrated pest management programs (AW-IPM) incorporating sterile insect technique (SIT) have been successful in suppressing populations of different fruit fly species during the last six decades. In addition, the development of genetic sexing strains (GSS) for different fruit fly species has allowed for sterile male-only releases and has significantly improved the efficacy and cost effectiveness of the SIT applications. The South American Fruit Fly Anastrepha fraterculus (Diptera: Tephritidae) is a major agricultural pest attacking several fruit commodities. This impedes international trade and has a significant negative impact on the local economies. Given the importance of sterile male-only releases, the development of a GSS for A. fraterculus would facilitate the implementation of an efficient and cost-effective SIT operational program against this insect pest species. For potential use in a GSS, three new morphological markers (mutants) were isolated in a laboratory strain of A. fraterculus sp. 1, including the black pupae (bp) gene located on chromosome VI. The black pupa phenotype was used as a selectable marker to develop genetic sexing strains by linking the wild type allele (bp+) to the Y-chromosome -via irradiation to induce a reciprocal Y-autosome translocation. Four GSS were established and one of them, namely GSS-89, showed the best genetic stability and the highest fertility. This strain was selected for further characterization and cytogenetic analysis. We herein report the development of the first genetic sexing strain of a major agricultural pest, A. fraterculus sp. 1, using as a selectable marker the black pupae genetic locus.

中文翻译：

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p的一种基于色genetic的遗传性别菌株的开发与鉴定。1（双翅目：T科）

在过去的六十年中，结合了不育昆虫技术（SIT）的区域性虫害综合治理计划（AW-IPM）已成功地抑制了不同果蝇物种的种群。此外，针对不同果蝇物种的遗传性别鉴定菌株（GSS）的开发已允许仅雄性不育的释放，并显着提高了SIT应用的功效和成本效益。南美果蝇Anastrestpha fraterculus（Diptera：Tephritidae）是一种主要的农业害虫，攻击几种水果商品。这阻碍了国际贸易，并对当地经济产生了重大的负面影响。鉴于仅雄性不育释放的重要性，因此开发了A的GSS。fraterculus将有助于针对该害虫物种实施有效且具有成本效益的SIT操作计划。为了在GSS中潜在使用，在实验室菌株A. fraterculus sp。中分离了三个新的形态学标记（突变体）。1，包括位于染色体VI上的黑p（bp）基因。黑表型被用作选择标记，通过通过辐射将野生型等位基因（bp +）与Y染色体连接，以诱导相互的Y常染色体易位，从而开发出遗传性别菌株。建立了四个GSS，其中一个GSS-89​​具有最佳的遗传稳定性和最高的育性。选择该菌株用于进一步表征和细胞遗传学分析。我们在此报告了主要农业害虫A的第一个遗传性别鉴定菌株的开发。fraterculus sp。1，使用黑using遗传基因座作为选择标记。