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Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37
Biotechnology & Biotechnological Equipment ( IF 1.4 ) Pub Date : 2020-12-17 , DOI: 10.1080/13102818.2020.1858722
Kun Guo 1, 2 , Qinyu Zhang 1, 2 , Jinyi Zhao 1, 2 , Zhu Li 1, 2 , Jiang Ran 1, 2 , Yang Xiao 3 , Su Zhang 1, 2 , Mianmian Hu 1, 2
Affiliation  

Abstract

A crude extract (B10) of Aspergillus niger spores showed effective antibacterial activity against Agrobacterium tumefaciens T-37. The inhibition rate was 98.22%, and the half maximal inhibitory concentration was 0.035 ± 0.018 mg/mL. To study the primary antibacterial mechanism, this study used relative electric conductivity, release of proteins and nucleic acids, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) and detection of reactive oxygen species (ROS). After adding B10, the relative electric conductivity increased in the supernatant indicating electrolyte transport from intracellular to extracellular space. Compared with the control group and the benzylpenicillin potassium (BP) group, the B10 group had a significant increase in extracellular nucleic acid and protein within 0–18 h, showing that the cell and cytoplasmic membranes were damaged by B10. The SDS–PAGE results confirmed that the extracellular protein and nucleic acid levels were in agreement with lower intracellular total protein. Finally, the intracellular levels of ROS indicated that B10 caused an increase in ROS. In summary, B10 showed clear antibacterial activity against A. tumefaciens through damaging cytoplasmic membranes. The research provided a scientific basis for the prevention and treatment of bacteria.



中文翻译:

黑曲霉xj孢子粉粗提物B10对根癌农杆菌T-37的抗菌作用

摘要

黑曲霉孢子的粗提物(B10)对根癌农杆菌具有有效的抗菌活性T-37。抑制率为98.22%,半数最大抑制浓度为0.035±0.018 mg / mL。为了研究主要的抗菌机制,该研究使用了相对电导率,蛋白质和核酸的释放,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和活性氧(ROS)的检测。添加B10后,上清液中的相对电导率增加,表明电解质从细胞内空间转移到细胞外空间。与对照组和苄青霉素钾(BP)组相比,B10组在0-18小时内细胞外核酸和蛋白质显着增加,表明细胞和细胞质膜被B10破坏。SDS-PAGE结果证实细胞外蛋白和核酸水平与较低的细胞内总蛋白一致。最后,ROS的细胞内水平表明B10引起ROS的增加。总之,B10对细菌具有明显的抗菌活性。根癌农杆菌通过破坏细胞质膜。该研究为细菌的防治提供了科学依据。

更新日期:2020-12-18
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