Abstract

A new α-pyrone derivative, namely annulopyronoate (1), together with the known isobenzofuranone, diphenyl ether, sesquiterpene, phenolic and diketopiperazine derivatives (2–12) were isolated from a filamentous fungus Annulohypoxylon stygium SWUF09-030. The structures were determined by analysis of spectroscopic and MS data. The absolute configuration of 1 was determined by comparison of experimental and calculated Electronic Circular Dichroism (ECD) spectra. Compounds 3–5 and 7 exhibited both anti-proliferative effects against HeLa, HT29, HCT116, MCF-7 and Vero cell lines and inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells.

摘要

一个新的α吡喃酮衍生物，即annulopyronoate（1）中，用已知的异苯并呋喃酮，二苯基醚，倍半萜烯，酚醛树脂和二酮哌嗪衍生物（共同2 - 12）从丝状真菌中分离Annulohypoxylon stygium SWUF09-030。通过光谱和MS数据分析确定结构。的绝对构型1，通过实验比较测定和计算电子圆二色性（ECD）光谱。化合物3 – 5和7 表现出对 HeLa、HT29、HCT116、MCF-7 和 Vero 细胞系的抗增殖作用，并抑制脂多糖 (LPS) 刺激的 RAW264.7 巨噬细胞中一氧化氮 (NO) 的产生。