Abstract

With the aim to establish a novel nano-formulation with a high nematocidal activity and safety for human as well as the ecosystem, in this work, lipid based nanoemulsions (LNs) co-encapsulating chili oil, cinnamon oil and neem oil (NaTri) was prepared by a combined method of homogenization and sonication. The mean particle size, polydispersity index (PDI), zeta potential of the prepared NaTri was 156.7 nm, 0.193 and −36.8 mV, respectively. NaTri showed a fast penetration into nematodes as well as plant roots, and it did not show any phytotoxicity. The presence of chili oil has showed a synergistic effect with cinnamon oil and neem oil resulting in a high nematocidal activity of NaTri. Particularly, its in vitro nematocidal activity at a 300 times dilution was 82.3%, while its ex vitro nematocidal activity at a 300 times dilution was more than 75% for the sprinkler irrigation and 91.3% for the drip irrigation. This novel nano-formulation shows great potential of wide application in sustainable horticulture to protect plants from the adverse effects of nematodes.

摘要

为了建立一种对人类和生态系统具有高杀线虫活性和安全性的新型纳米制剂，在这项工作中，脂质基纳米乳液 (LNs) 共包封辣椒油、肉桂油和印em 油 (NaTri)采用均质和超声联合方法制备。制备的 NaTri 的平均粒径、多分散指数 (PDI) 和 zeta 电位分别为 156.7 nm、0.193 和 -36.8 mV。NaTri 显示出对线虫和植物根部的快速渗透，并且没有显示出任何植物毒性。辣椒油的存在显示出与肉桂油和印楝油的协同作用，导致 NaTri 具有高杀线虫活性。特别地，其体外杀线虫活性在 300 倍稀释时为 82.3%，而其体外喷灌300倍杀线虫活性大于75%，滴灌为91.3%。这种新型纳米制剂显示出在可持续园艺中广泛应用的巨大潜力，可以保护植物免受线虫的不利影响。