As sequencing and data analysis methods improve and more bacterial species are identified, microbial profiling has been performed more widely in marine species. To enable the development of potential prophylactic strategies against bacterial disease in abalone induced by elevated water temperatures, the left kidney and stomach samples of six unaffected adult greenlip abalone Haliotis laevigata were sampled in summer and winter to enable comparisons of endogenous abalone microbial profiles. Under the experimental conditions, needle aspiration of selected tissues did not allow collection of adequate amounts of abalone tissue for microbiome studies. Tenericutes, Proteobacteria and Fusobacteria were the three major phyla present in most samples which were previously identified in other marine species. Propionibacterium, Psychrilyobacter, Pseudoruegeria, Ruegeria, Arcobacter, Shewanella, Pseudoalteromonas, Streptococcus and Mycoplasma were the nine genera that were detected in 0.5% or more of all samples. The beta‐diversity results indicated there were significant differences between summer and winter samples. The microbiome structures were different between left kidney and stomach samples. It was found that the abalone left kidney harboured diverse bacteria and was a potential indicator of the physiological status of the abalone, offering an alternative site for microbial profiling of abalone, apart from the gastrointestinal tract.

中文翻译：

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养殖成年绿唇鲍鱼（Haliotis laevigata）左肾和胃的季节性微生物分布

随着测序和数据分析方法的改进以及更多细菌种类的发现，在海洋物种中微生物分布图的应用越来越广泛。为了开发针对因水温升高引起的鲍鱼细菌性疾病的潜在预防策略，在夏季和冬季对六个未受影响的成年绿唇鲍鱼Haliotis laevigata的左肾和胃样品进行了采样，以比较内源性鲍鱼的微生物特征。在实验条件下，选定组织的针吸法不能收集足够量的鲍鱼组织用于微生物组研究。Tenericutes，Proteobacteria和Fusobacteria是大多数样品中存在的三个主要门，这些样品先前已在其他海洋物种中鉴定出来。丙酸，Psychrilyobacter，Pseudoruegeria，Ruegeria，弓形杆菌，希瓦氏菌属，假交替单胞，链球菌和支原体分别在0.5％，检测以上所有样品的该9个属。β多样性结果表明，夏季和冬季样品之间存在显着差异。左肾和胃样品之间的微生物组结构不同。发现鲍鱼左肾中携带着多种细菌，是鲍鱼生理状态的潜在指标，除了胃肠道外，还为鲍鱼的微生物剖析提供了另一个场所。