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A rice tubulin tyrosine ligase‐like 12 protein affects the dynamic and orientation of microtubules
Journal of Integrative Plant Biology ( IF 11.4 ) Pub Date : 2020-12-18 , DOI: 10.1111/jipb.13059
Kunxi Zhang 1 , Xin Zhu 1 , Steffen Durst 1 , Petra Hohenberger 1 , Min-Jung Han 2 , Gynheung An 3 , Vaidurya P Sahi 1 , Michael Riemann 1 , Peter Nick 1
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The detyrosination/retyrosination cycle is the most common post‐translational modification of α‐tubulin. Removal of the conserved C‐terminal tyrosine of α‐tubulin by a still elusive tubulin tyrosine carboxypeptidase, and religation of this tyrosine by a tubulin tyrosine ligase (TTL), are probably common to all eukaryotes. Interestingly, for plants, the only candidates qualifying as potential TTL homologs are the tubulin tyrosine ligase‐like 12 proteins. To get insight into the biological functions of these potential TTL homologs, we cloned the rice TTL‐like 12 protein (OsTTLL12) and generated overexpression OsTTLL12‐RFP lines in both rice and tobacco BY‐2 cells. We found, unexpectedly, that overexpression of this OsTTLL12‐RFP increased the relative abundance of detyrosinated α‐tubulin in both coleoptile and seminal root, correlated with more stable microtubules. This was independent of the respective orientation of cortical microtubule, and followed by correspondingly changing growth of coleoptiles and seminal roots. A perturbed organization of phragmoplast microtubules and disoriented cell walls were further characteristics of this phenotype. Thus, the elevated tubulin detyrosination in consequence of OsTTLL12 overexpression affects structural and dynamic features of microtubules, followed by changes in the axiality of cell plate deposition and, consequently, plant growth.

中文翻译:

水稻微管蛋白酪氨酸连接酶样 12 蛋白影响微管的动态和方向

去酪氨酸化/再酪氨酸化循环是α-微管蛋白最常见的翻译后修饰。通过仍然难以捉摸的微管蛋白酪氨酸羧肽酶去除α-微管蛋白的保守 C 端酪氨酸,并通过微管蛋白酪氨酸连接酶 (TTL) 重新连接该酪氨酸,这可能对所有真核生物都很常见。有趣的是,对于植物,唯一有资格作为潜在 TTL 同源物的候选物是微管蛋白酪氨酸连接酶样 12 蛋白。为了深入了解这些潜在 TTL 同源物的生物学功能,我们克隆了水稻 TTL 样 12 蛋白(OsTTLL12)并在水稻和烟草 BY-2 细胞中产生了过表达 OsTTLL12-RFP 系。我们意外地发现,这种 OsTTLL12-RFP 的过度表达增加了去酪氨酸的相对丰度。胚芽鞘和精根中的α-微管蛋白,与更稳定的微管相关。这与皮层微管各自的方向无关,随后胚芽鞘和精根的生长相应地发生变化。这种表型的进一步特征是成膜微管的扰动组织和方向错乱的细胞壁。因此,OsTTLL12 过表达导致的微管蛋白去酪氨酸化升高影响微管的结构和动态特征,随后细胞板沉积的轴向发生变化,从而影响植物生长。
更新日期:2020-12-18
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