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Population genetic analysis of lettuce big-vein disease viruses and their vector fungi Olpidium virulentus in Ankara province, Turkey
Physiological and Molecular Plant Pathology ( IF 2.7 ) Pub Date : 2020-12-18 , DOI: 10.1016/j.pmpp.2020.101593
Filiz Randa Zelyüt , Filiz Ertunç

Lettuce big-vein disease (LBVD) is caused by either Mirafiori lettuce big-vein virus (MiLBVV) alone, or co-infected with Lettuce big-vein associated virus (LBVaV). Both viruses are transmitted by the fungal vector Olpidium spp. In the present study, population genetics of these two viruses and their fungal vector, O. virulentus, were analyzed, using the nucleotide sequences of the virus coat protein (CP) partial genes and the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) of the vector fungi. The analysis was conducted along with all previously reported LBVD viruses and Olpidium spp. populations. We used conventional PCR techniques to identify the viruses in lettuce samples and their fungal vector in soil samples collected from the root zone of field-grown lettuce plants in the Ankara province of Turkey. Phylogenetic analyses showed that LBVD viruses had at least two evolutionary lineages, and Ankara LBVD isolates were more closely related to the European populations. Also, we found that there is a strong negative selection pressure in the partial CP genes of LBVD viruses isolated from Ankara, due to the effective terrestrial climate. The negative selection pressure indicates balancing selection within Turkish isolates and other populations. The population of O. virulentus isolates from Ankara and other geographic regions were branched into five sub-clades, and there was no geographical separation in these isolates. Our analysis also showed Olpidium spp. to be monophyletic, and showed speciation among the isolates.



中文翻译:

土耳其安卡拉省生菜大静脉病病毒及其介导的真菌Olpidium v​​irulentus的种群遗传分析

生菜大静脉疾病(LBVD)是由Mirafiori生菜大静脉病毒(MiLBVV)单独引起的,或与生菜大静脉相关病毒(LBVaV)共同感染。两种病毒均通过真菌载体Olpidium spp传播。在本研究中,使用病毒外壳蛋白(CP)部分基因的核苷酸序列和核糖体DNA的内部转录间隔区(ITS)区,分析了这两种病毒及其真菌载体O. virulentus的种群遗传学。rDNA)的载体真菌。与先前报告的所有LBVD病毒和Olpidium一起进行了分析spp。人口。我们使用常规PCR技术鉴定了从土耳其安卡拉省田地生菜植物根部收集的生菜样品中的病毒及其土壤样品中的真菌载体。系统发育分析表明,LBVD病毒至少具有两个进化谱系,而安卡拉LBVD分离株与欧洲人群关系更密切。此外,我们发现由于有效的陆地气候,从安卡拉分离出的LBVD病毒的部分CP基因存在强烈的负选择压力。负的选择压力表明在土耳其隔离群和其他人群中的平衡选择。O. virulentus的人口来自安卡拉和其他地理区域的分离株被分为五个亚属,这些分离株没有地理上的分离。我们的分析还显示了Olpidium spp。是单系的,并且在分离物中显示出物种。

更新日期:2020-12-25
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