Jerricans are commonly used to store household drinking water. However, biofilms can grow on jerrican surfaces and contaminate water. To investigate mechanisms to inhibit biofilm growth, 72 5 L jerricans containing E. coli-spiked water were incubated for 10 weeks in the laboratory. Jerricans were stratified by: water treatment with chlorine; turbidity; and, regular cleaning with NaOCl, rocks, sand, NaOCl/rocks, and NaOCl/sand. At study end, surface E. coli was enumerated and biofilms imaged using epifluorescence microscopy; free chlorine residual was tested regularly. We found biofilms grew rapidly in jerricans (presence confirmed in 3 weeks), and chlorine demand within jerricans increased over time as FCR decreased from 1.16 to 0.59 mg L⁻¹ over the course of the study in 5 NTU treated waters. Biofilm growth was significantly inhibited by water treatment with chlorine (p = 0.03), and was inhibited by cleaning with NaOCl or NaOCl/rocks. Cleaning with rocks increased surface roughness, which promotes biofilm growth. These results highlight the need for research on locally-appropriate cleaning methods and/or alternatives (e.g. replacement, biofilm-resistant surfaces) to ensure jerricans provide safe storage of drinking water.

中文翻译：

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本地可用的清洁方法和家庭加氯对抑制用于储存家庭饮用水的豆类中生物膜形成的功效

喷气式飞机通常用于存储家庭饮用水。但是，生物膜可能会在干胶表面上生长并污染水。为了研究抑制生物膜生长的机制，在实验室中将72 5 L的含有大肠埃希菌加标水的豆浆培养10周。飓风的分类依据是：氯水处理；浊度 并定期用NaOCl，岩石，沙子，NaOCl /岩石和NaOCl /砂子进行清洁。在研究结束时，对表面大肠杆菌进行计数，并使用落射荧光显微镜对生物膜成像；定期测试残留的游离氯。我们发现，在Jerricans中，生物膜迅速增长（在3周内确认存在），并且随着FCR从1.16降至0.59 mg L ^-1， Jerricans中的氯需求随时间增加。在5 NTU处理过的水中进行研究的过程中。生物膜的生长受到氯水处理的显着抑制（p = 0.03），并被NaOCl或NaOCl /岩石清洗抑制。用岩石清洁会增加表面粗糙度，从而促进生物膜的生长。这些结果突出表明，需要研究适合当地的清洁方法和/或替代方法（例如，更换，抗生物膜的表面），以确保豆类食品提供安全的饮用水储存。