An ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for simultaneous determination of 15 mycotoxins, including aflatoxins (B₁, B₂, G₁, and G₂), ochratoxins (A, B, and C), citrinin, patulin, and emerging Alternaria toxins (alternariol, alternariol monomethyl ether, altenuene, tentoxin, tenuazonic acid, and altenusin) in orange, grape and apple juices. Different extraction approaches, sorbents, chromatographic columns and mobile phases were investigated for establishment of an optimal QuEChERS procedure and UHPLC-MS/MS conditions. Recoveries were in the range of 74–110%, and the limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.05 to 0.1 ng mL⁻¹ and from 0.1 to 5.0 ng mL⁻¹, respectively. Matrix effects were evaluated and matrix-matched calibration curves were used to compensate for matrix effects and achieve accurate quantification. The correlation coefficients (R²) of linearity were higher than 0.99 and the relative standard deviations (RSDs) of intra- and inter-day precision were under 13%. The method was subsequently applied to 22 fruit juice samples. The high frequencies (90.9%) of mycotoxins not only proved the reliability and sensitivity of the currently established method, but also demonstrated that fruit juices are susceptible to different mycotoxins, which need to be continuously monitored in the future.

中文翻译：

---

一种可靠，准确的UHPLC-MS / MS方法，用于筛查橙汁，葡萄汁和苹果汁中的曲霉菌，青霉菌和链霉菌真菌毒素

开发了一种超高效液相色谱-串联质谱（UHPLC-MS / MS）方法，用于同时测定15种霉菌毒素，包括黄曲霉毒素（B ₁，B ₂，G ₁和G ₂），曲霉毒素（A，B和C），柑桔素，展青霉素和新兴的链格孢菌橙汁，葡萄汁和苹果汁中的毒素（alternariol，alternariol单甲醚，altenuene，tentoxin，tenuazonic酸和altenusin）。为了建立最佳的QuEChERS程序和UHPLC-MS / MS条件，研究了不同的提取方法，吸附剂，色谱柱和流动相。回收率在74–110％的范围内，检出限（LOD）和定量限（LOQ）分别为0.05至0.1 ng mL ^-1和0.1至5.0 ng mL ^-1。评估基质效应，并使用基质匹配的校准曲线补偿基质效应并实现准确定量。相关系数（R ²）线性度高于0.99，日内和日间精度的相对标准偏差（RSD）低于13％。该方法随后应用于22个果汁样品。真菌毒素的高频率（90.9％）不仅证明了目前建立的方法的可靠性和敏感性，而且还表明果汁易受不同真菌毒素的影响，将来需要对其进行连续监测。