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Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications
Journal of Bioactive and Compatible Polymers ( IF 1.7 ) Pub Date : 2020-12-16 , DOI: 10.1177/0883911520973244
Xin Liu 1 , Bo Chen 2 , Yan Li 1 , Yan Kong 3, 4 , Ming Gao 3, 4 , Lu Zhong Zhang 3, 4 , Ning Gu 1
Affiliation  

Long-distance (⩾10 mm) arterial vascular defect injury was a massive challenge affecting human health. Compared with autologous transplantation, tissue-engineered scaffolds such as biocompatible silk fibroin (SF) scaffolds have been developed because they exhibit equivalent functional repair effects without adverse reactions. However, its mechanical strength and structural stability needed to be further improved to match the longer repair cycle of blood vessels while maintaining the original biological safety. Hence, we designed and prepared SF and hydrophobic polycaprolactone (PCL) composite microfibers by an improving electrospinning method. It was found that when the weight ratio of PCL to SF was 1: 1, a microfiber scaffold with high strength (6.16 N) and minimum degradability can be obtained. More importantly, compared with natural silk fibroin, the novel composite microfiber scaffolds can slightly inhibit cell infiltration and inflammation through co-culture with HUVECs in vitro and rabbit back transplantation in vivo. Furthermore, the fabricated scaffolds also demonstrated excellent structural stability in vivo because of the well-organized PCL doping in the structure. All these results indicated that the novel PCL/SF composite microfiber scaffolds were promising candidates for vascular tissue engineering applications.

中文翻译:

开发用于潜在血管组织工程应用的电纺聚己内酯/丝绸支架

长距离(⩾10 mm)动脉血管缺损损伤是影响人类健康的巨大挑战。与自体移植相比,组织工程支架如生物相容的丝素蛋白 (SF) 支架已被开发出来,因为它们表现出等效的功能修复效果而没有不良反应。然而,其机械强度和结构稳定性需要进一步提高,以适应更长的血管修复周期,同时保持原有的生物安全性。因此,我们通过改进的静电纺丝方法设计并制备了 SF 和疏水性聚己内酯 (PCL) 复合微纤维。结果发现,当PCL与SF的重量比为1:1时,可以获得强度高(6.16 N)、降解性最小的超细纤维支架。更重要的是,与天然丝素蛋白相比,新型复合超细纤维支架通过体外与HUVECs共培养和兔体内回移植可以轻微抑制细胞浸润和炎症。此外,由于结构中组织良好的 PCL 掺杂,制造的支架在体内也表现出优异的结构稳定性。所有这些结果表明,新型 PCL/SF 复合微纤维支架是血管组织工程应用的有希望的候选者。
更新日期:2020-12-16
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