One of the most important challenges in the field of microfluidics is the rapid fabrication of microchips with complex topologies. Although the processing method of microfluidic chips has made brilliant achievements in the past 20 years, almost all traditional processing methods still face huge obstacles in the production of complex topologies and three-dimensional microchannel. Nowadays, the main methods of manufacturing microfluidic chips such as numerical control microprocessing, laser ablation, inkjet printing, photolithography, dry etching, and lithography, galvanoformung and abformung (LIGA) technology are not only inapplicable to the complex topological structure and the rapid processing of three-dimensional microfluidic chips but also rely on expensive processing equipment, complex manufacturing process, and low yield. To solve the problems of these traditional processing methods, we propose a low-cost methodology to obtain a microfluidic chip by sewing the chip pipe to the substrate with an embroidery machine as low as $6. Compared with the above-mentioned traditional microprocessing technologies, the new chip processing technology proposed by us does not involve professional microprocessing equipment and professional skills. Therefore, this new chip processing technology can significantly improve the efficiency of microprocessing.

中文翻译：

---

复杂拓扑结构和三维微通道的用户友好的微细加工方法及其在聚合酶链反应（PCR）中的应用前景

微流体领域中最重要的挑战之一是快速制造具有复杂拓扑的微芯片。尽管微流控芯片的加工方法在过去的20年中取得了辉煌的成就，但几乎所有传统的加工方法在复杂的拓扑结构和三维微通道的生产中仍然面临着巨大的障碍。如今，制造微流控芯片的主要方法，例如数控微处理，激光烧蚀，喷墨印刷，光刻，干法蚀刻和光刻，电化和电化（LIGA）技术不仅不适用于复杂的拓扑结构和快速的加工工艺。三维微流控芯片还依赖于昂贵的加工设备，复杂的制造工艺和低产量。为了解决这些传统加工方法的问题，我们提出了一种低成本的方法，可通过使用绣花机将芯片管缝到基材上来将芯片管缝到基材上，从而获得微流体芯片。与上述传统微处理技术相比，我们提出的新型芯片处理技术不涉及专业的微处理设备和专业技能。因此，这种新的芯片处理技术可以显着提高微处理效率。我们提出的新芯片处理技术不涉及专业的微处理设备和专业技能。因此，这种新的芯片处理技术可以显着提高微处理效率。我们提出的新芯片处理技术不涉及专业的微处理设备和专业技能。因此，这种新的芯片处理技术可以显着提高微处理效率。