Exposure of Eucalyptus tree stems to the radiant heat of forest fires can kill cambial cells and their embedded regenerative meristems, thus preventing epicormic resprouting and recovery of the tree. Currently there is no tissue-level method to quantify the viability of cambial cells in Eucalyptus following heat exposure. The first aim of this study was to adapt and validate the tetrazolium reduction method of testing for cell viability in Eucalyptus. The second aim was to apply the method to establish a threshold level of cambium cell viability in Eucalyptus obliqua to enable the identification of a critical temperature.

中文翻译：

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桉树茎血管形成层和次生韧皮部热诱导细胞坏死的定量测试

桉树的茎部暴露于森林大火的辐射热中会杀死冈比亚细胞及其内嵌的再生分生组织，从而阻止了树皮的表皮再生和恢复。目前，尚无组织水平的方法来量化热暴露后桉树中冈比亚细胞的活力。这项研究的第一个目的是适应和验证四唑还原法检测桉树细胞活力的方法。第二个目的是应用该方法建立斜纹桉树中形成层细胞活力的阈值水平，从而能够确定临界温度。