Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 μg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.

中文翻译：

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抗菌磁性聚（GMA）微粒：合成，表征和溶菌酶固定化

目前，微米大小的磁性颗粒在许多领域都有广泛的应用，包括生物技术，生物化学，胶体科学和医学。在这项研究中，通过在Fe（II）-Ni（II）磁性复盐周围提供聚合来合成磁性聚甲基丙烯酸缩水甘油酯微粒。用这些颗粒研究了水系统中溶菌酶蛋白的吸附。在不断变化的pH值，可变量的吸附剂，不同的相互作用时间和溶菌酶量的条件下进行吸附研究。研究了颗粒的吸附能力，并且获得了约95.6mg溶菌酶/ g微粒的值。检查了固定的溶菌酶的酶活性，发现与游离酶相比更稳定和可重复使用。固定的酶在运行五次后仍显示80％的活性，并在60天结束时设法保持其初始活性的78％。此外，在对六种不同微生物的抗微生物分析研究中，与游离溶菌酶一样，溶菌酶固定化颗粒的最小抑菌浓度值计算为125μg/ mL。最后，发现吸附相互作用与朗缪尔等温线模型兼容。因此，可以说磁性聚（GMA）微粒是用于溶菌酶固定的合适材料，并且固定化的溶菌酶可以用于生物技术研究。与游离溶菌酶一样，溶菌酶固定颗粒的最小抑菌浓度值为125μg/ mL。最后，发现吸附相互作用与朗缪尔等温线模型兼容。因此，可以说磁性聚（GMA）微粒是用于溶菌酶固定的合适材料，并且固定化的溶菌酶可以用于生物技术研究。与游离溶菌酶一样，溶菌酶固定颗粒的最小抑菌浓度值为125μg/ mL。最后，发现吸附相互作用与朗缪尔等温线模型兼容。因此，可以说磁性聚（GMA）微粒是用于溶菌酶固定的合适材料，并且固定化的溶菌酶可以用于生物技术研究。