Rotaviruses are among the major causes of viral acute gastroenteritis in newborns and children younger than 5 years worldwide. The ability of rotaviruses to remain infectious in harsh environments as well as in the wastewater treatment process makes them one of the most prevalent enteric viruses. The current study aimed to determine the presence of rotavirus genomes and to analyze them phylogenetically in secondary treated wastewater (TW) samples. In total, 13 TW samples were collected from September 2017 to August 2018. Viral concentration was carried out using the absorption-elution method, and after RNA extraction and cDNA synthesis, real-time and conventional polymerase chain reaction (PCR) were performed. A phylogenetic tree was drawn using Maximum Likelihood and Tamura 3-parameter using MEGA v.6 software. Rotavirus genomes were detected in 7/13 (53.8%) and 3/13 (23.07%) samples using reverse transcription (RT)-PCR and conventional PCR, respectively. Accordingly, phylogenetic analysis revealed G4P[8], G9P[4], and G9P[8] genotypes among the samples. The presence of rotavirus in secondary TW samples discharged into surface water emphasizes the importance of monitoring and assessing viral contamination in the water sources used for agricultural and recreational purposes.

轮状病毒是全世界新生儿和5岁以下儿童急性病毒性肠胃炎的主要原因。轮状病毒在恶劣环境以及废水处理过程中仍具有传染性，这使其成为最流行的肠病毒之一。当前的研究旨在确定轮状病毒基因组的存在，并进行系统分析，以分析二级处理废水（TW）样品中的轮状病毒。从2017年9月至2018年8月，总共收集了13份TW样品。使用吸收-洗脱方法进行病毒浓缩，并在RNA提取和cDNA合成后进行实时和常规聚合酶链反应（PCR）。使用MEGA v.6软件使用Maximum Likelihood和Tamura 3参数绘制系统发育树。使用逆转录（RT）-PCR和常规PCR分别在7/13（53.8％）和3/13（23.07％）样品中检测到轮状病毒基因组。因此，系统发育分析显示样品中有G4P [8]，G9P [4]和G9P [8]基因型。排入地表水中的次级TW样品中存在轮状病毒，这强调了监测和评估用于农业和娱乐目的的水源中病毒污染的重要性。