Jordan is one of the lowest countries in the world in terms of water resources. The reuse of treated wastewater is an important alternative to supply agricultural demands for water. In Jordan, Kherbet Al-Samra wastewater treatment plant (KSWWTP) is the largest and its effluent is mainly used for irrigation purposes. In this study, bacterial contamination and mutagenic potential were evaluated in six sites, beginning with KSWWTP and ending with King Tallal Dam. The results showed high contamination with many pathogenic bacteria and coliforms. The isolated pathogenic bacteria were Salmonella sp., Shigella sp., Bacillus cereus and Staphylococcus aureus. The isolated opportunistic pathogenic bacteria were Acinetobacter lwoffii, Elizabethkingia meningosepticum, Pseudomonas fluorescens and Bacillus licheniformis. These bacteria were found in all sampling sites without a specific prevalence pattern. Differences in temperature between seasons affect total coliform and other bacterial count. All water samples showed positive mutagenic activity and high bacterial pollution. Improving the disinfection efficiency in the wastewater treatment plant is important to minimize potential toxicity and exposure of public health to pathogenic bacteria, reduce water resources' contamination and environmental pollution. Increasing effluent sampling frequency from KSWWTP is required to monitor bacterial contamination and toxicity/mutagenicity level for water safety and public health risk assessments.

就水资源而言，约旦是世界上最低的国家之一。处理后的废水回用是满足农业用水需求的重要替代方案。在约旦，Kherbet Al-Samra废水处理厂（KSWWTP）是最大的，其废水主要用于灌溉目的。在这项研究中，评估了六个地点的细菌污染和诱变潜力，从KSWWTP开始到塔拉勒国王大坝结束。结果表明，许多致病细菌和大肠菌均高度污染。分离的致病细菌是沙门氏菌，志贺氏菌，蜡状芽孢杆菌和金黄色葡萄球菌。分离出的机会致病菌是沃氏不动杆菌，脑膜炎伊丽莎白女王，荧光假单胞菌和地衣芽孢杆菌。这些细菌在所有采样点均未发现特定的流行模式。季节之间的温度差异会影响大肠菌群和其他细菌的总数。所有水样均显示出积极的诱变活性和高细菌污染。提高废水处理厂的消毒效率，对于最大程度地减少潜在的毒性和使公共卫生暴露于病原细菌，减少水资源污染和环境污染至关重要。为了监测细菌污染和毒性/致突变性水平，需要对KSWWTP的废水采样频率进行提高，以进行水安全和公共卫生风险评估。