Abstract

Background

We developed a novel balloon-based endobiliary radiofrequency ablation (RFA) system to overcome the limitations of conventional RFA in treating malignant biliary strictures. This study aimed to evaluate the histological effects of balloon-RFA in porcine bile ducts.

Methods

Balloon-RFA of intrahepatic bile ducts from freshly resected porcine livers was performed for 60 s at 50, 60 and 70 °C. The specimens were subsequently sectioned for histological analysis and the slides were digitally scanned. Pseudo-coloring was applied based on the intensity of the ablation effect, and the area and estimated volume of ablation were measured.

Results

All samples showed epithelial sloughing and coagulative necrosis, and the entire internal circumference of the bile duct was evenly ablated. Complete transmural necrosis and involvement beyond the wall were found in 0% of samples ablated at 50 °C, in 33% at 60 °C and 100% at 70 °C. The mean depth and estimated volume of the ablation areas were 1.29 mm and 353.84 mm³, 1.44 mm, and 505.54 mm³ and 1.81 mm and 702.52 mm³ at 50, 60 and 70 °C, respectively.

Conclusions

Balloon-RFA leads to sloughing of the mucosa and coagulative necrosis. Ablation depth and volume increased in proportion to target temperature.

中文翻译：

---

评估猪胆囊内球囊内胆道射频消融的组织作用

摘要

背景

我们开发了一种新颖的基于气球的胆道内射频消融（RFA）系统，以克服常规RFA在治疗恶性胆道狭窄中的局限性。本研究旨在评估球囊-RFA在猪胆管中的组织学作用。

方法

在50、60和70°C下，对刚切除的猪肝脏的肝内胆管进行气囊RFA检查60 s。随后将标本切片以进行组织学分析，并对载玻片进行数字扫描。根据消融效果的强度应用伪彩色，并测量消融的面积和估计的体积。

结果

所有样品均显示出上皮脱落和凝血坏死，并且胆管的整个内周被均匀地消融。在50°C烧蚀的样品中，有0％的样品出现了完全的透壁坏死并累及壁外，在60°C的样品中发现了33％，在70°C的样品中发现了100％。消融区域的平均深度和估计的体积分别为1.29毫米和353.84毫米³，1.44毫米，505.54毫米³和1.81毫米和702.52毫米³分别在50,60和70℃。

结论

球囊RFA导致粘膜脱落和凝血坏死。烧蚀深度和体积与目标温度成正比。