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Antioxidant action of vitamin E in vivo as assessed from its reaction products with multiple biological oxidants
Free Radical Research ( IF 3.3 ) Pub Date : 2020-12-16 , DOI: 10.1080/10715762.2020.1866181
Etsuo Niki 1 , Noriko Noguchi 2
Affiliation  

Abstract

Vitamin E acts as essential antioxidant against detrimental oxidation of biological molecules induced by multiple reactive species. To gain more insight into the physiological role of vitamin E, the levels of its oxidation products in humans under normal and pathological conditions were compared. α-Tocopherol quinone (α-TQ) and 5-nitro-γ-tocopherol (5-NgT) were focused. α-TQ is produced by multiple oxidants including oxygen radicals, peroxynitrite, hypochlorite, singlet oxygen, and ozone, while 5-NgT is produced by nitrogen dioxide radical derived from peroxynitrite and the reaction of nitrite and hypochlorite. The reported concentrations of α-TQ and 5-NgT in healthy human plasma are highly variable ranging from 15 to 360 and 4 to 170 nM, respectively. In general, the molar ratio 5-NgT/γ-tocopherol was higher than the ratio α-TQ/α-tocopherol. Both absolute concentrations of α-TQ and 5-NgT and the molar ratios to the parent tocopherols were elevated significantly in the plasma of patients with various diseases compared with healthy subjects except neurological diseases. The molar ratios of the products to the respective parent compounds decreased in the order of 5-NgT/γ-tocopherol > α-TQ/α-tocopherol > hydroxyoctadecadienoate/linoleate >3-nitrotyrosine/tyrosine > isoprostane/arachidonate. The molar ratios of nitrated products to the respective parent compounds in human plasma are approximately 10−2 for 5-NgT and 10−5 for 3-nitrotyrosine, nitro-oleic acid, and 8-nitroguaine. These data indicate that vitamin E acts as an important physiological antioxidant and that α-TQ and 5-NgT represent biomarker for oxidative stress and nitrative stress respectively. (232 words)



中文翻译:

从其与多种生物氧化剂的反应产物评估维生素 E 在体内的抗氧化作用

摘要

维生素 E 是一种必需的抗氧化剂,可对抗由多种活性物质引起的生物分子的有害氧化。为了更深入地了解维生素 E 的生理作用,我们比较了正常和病理条件下人体中其氧化产物的水平。重点关注 α-生育酚醌 (α-TQ) 和 5-硝基-γ-生育酚 (5-NgT)。α-TQ 是由氧自由基、过氧亚硝酸盐、次氯酸盐、单线态氧和臭氧等多种氧化剂产生的,而 5-NgT 是由过氧亚硝酸盐衍生的二氧化氮自由基与亚硝酸盐和次氯酸盐反应产生的。健康人血浆中报告的 α-TQ 和 5-NgT 浓度变化很大,分别为 15 至 360 和 4 至 170 nM。一般来说,5-NgT/γ-生育酚的摩尔比高于α-TQ/α-生育酚的摩尔比。与除神经系统疾病外的健康受试者相比,患有各种疾病的患者血浆中α-TQ和5-NgT的绝对浓度以及与母体生育酚的摩尔比均显着升高。产物与各自母体化合物的摩尔比按 5-NgT/γ-生育酚 > α-TQ/α-生育酚 > 羟基十八碳二烯酸酯/亚油酸酯 > 3-硝基酪氨酸/酪氨酸 > 异前列烷/花生四烯酸酯的顺序降低。人血浆中硝化产物与相应母体化合物的摩尔比约为 10 产物与各自母体化合物的摩尔比按 5-NgT/γ-生育酚 > α-TQ/α-生育酚 > 羟基十八碳二烯酸酯/亚油酸酯 > 3-硝基酪氨酸/酪氨酸 > 异前列烷/花生四烯酸酯的顺序降低。人血浆中硝化产物与相应母体化合物的摩尔比约为 10 产物与各自母体化合物的摩尔比按 5-NgT/γ-生育酚 > α-TQ/α-生育酚 > 羟基十八碳二烯酸酯/亚油酸酯 > 3-硝基酪氨酸/酪氨酸 > 异前列烷/花生四烯酸酯的顺序降低。人血浆中硝化产物与相应母体化合物的摩尔比约为 10-2用于 5-NgT,10 -5用于 3-硝基酪氨酸、硝基油酸和 8-硝基胍。这些数据表明,维生素 E 是一种重要的生理抗氧化剂,α-TQ 和 5-NgT 分别代表氧化应激和硝化应激的生物标志物。(232 字)

更新日期:2020-12-17
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