Foot-and-mouth disease virus (FMDV) has led to serious losses in animal husbandry worldwide. Seromonitoring of FMDV postvaccination is important for the control and eradication of foot-and-mouth disease (FMD) in regions and countries where vaccination is widespread. However, many commercial kits present high false-positive rates. In this study, a multiepitope-based indirect chemiluminescence immunoassay (ME-CLIA) was developed for specifically detecting antibodies against FMDV serotype O in swine sera. The developed method presented high diagnostic sensitivity and excellent diagnostic specificity, and it could detect a broad spectrum of antibodies against FMDV serotype O. The diagnostic performance, accuracy rate, and analytical sensitivity of ME-CLIA were compared with those of three commercial kits. The immune protection value of multiple-epitope recombinant vaccine detected using ME-CLIA was preliminarily determined by observation of clinical symptoms postimmunization challenge, the results of which indicated that the ME-CLIA can be employed as a matching detection method for evaluating multiple-epitope recombinant vaccine. The percent positive values of ME-CLIA determined using swine vaccinated with inactivated vaccine were significantly positively correlated with the titers of liquid-phase-blocking enzyme-linked immunosorbent assay (ELISA) (LBPE) (r = 0.8361; P < 0.0001). These results indicated that ME-CLIA is suitable for detection of antibodies against FMDV serotype O in swine and for potency evaluation of multiple-epitope and inactivated vaccines.

中文翻译：

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使用多表位重组蛋白特异性检测猪口蹄疫病毒血清型 O 型抗体的间接化学发光免疫测定法的开发

口蹄疫病毒（FMDV）已导致全球畜牧业遭受严重损失。在疫苗接种广泛的地区和国家，口蹄疫疫苗接种后的血清监测对于控制和根除口蹄疫 (FMD) 很重要。然而，许多商业试剂盒的假阳性率很高。在这项研究中，开发了一种基于多表位的间接化学发光免疫分析 (ME-CLIA)，用于特异性检测猪血清中针对口蹄疫病毒 O 型的抗体。所开发的方法具有较高的诊断灵敏度和优良的诊断特异性，可以检测广谱的抗口蹄疫病毒 O 型抗体。将 ME-CLIA 的诊断性能、准确率和分析灵敏度与三种商业试剂盒进行比较。通过观察免疫激发后的临床症状，初步确定了ME-CLIA检测的多表位重组疫苗的免疫保护价值，结果表明ME-CLIA可作为评价多表位重组疫苗的匹配检测方法。疫苗。接种灭活疫苗的猪测定的 ME-CLIA 阳性百分比值与液相阻断酶联免疫吸附试验 (ELISA) (LBPE) 的滴度呈显着正相关。r = 0.8361；P < 0.0001)。这些结果表明ME-CLIA适用于检测猪中抗FMDV O血清型的抗体以及用于多表位和灭活疫苗的效力评估。