Bacteria survive metal stress by several mechanisms and metal binding is one such mechanism which has been screened in the present study to investigate the survival strategies of metal resistant bacteria. The production of siderophores, a metal chelating agent, was detected by chrome azurol S agar assay. The changes in cell wall studied by analyzing the peptidoglycan and teichoic acid content indicated an increase in the cell wall content. Evaluation of morphological and physiological alterations like cell size, granularity analyzed by SEM and flow cytometry analysis revealed an increase in cell size and granularity respectively. The transformation of phosphates monitored by 31 P NMR analysis indicated the presence of inorganic phosphate. Based on the cell wall changes and the 31 P NMR analysis, the surface charge of the organism was studied by zeta potential which displayed a difference at pH7.

中文翻译：

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铬结合蜡样芽孢杆菌 VITSH1-重金属清除的有希望的候选者

细菌通过多种机制在金属胁迫中存活下来，金属结合是一种这样的机制，在本研究中已筛选出一种机制，以研究金属抗性细菌的生存策略。铁载体（一种金属螯合剂）的产生通过铬天青 S 琼脂测定法检测。通过分析肽聚糖和磷壁酸含量研究的细胞壁变化表明细胞壁含量增加。对形态学和生理学改变的评估，如细胞大小、SEM 分析的粒度和流式细胞术分析显示，细胞大小和粒度分别增加。通过 31 P NMR 分析监测的磷酸盐的转化表明存在无机磷酸盐。根据细胞壁变化和 31 P NMR分析，