Waterbodies such as lakes and ponds are fragile environments affected by human influences. Suitable conditions can result in massive growth of phototrophs, commonly referred to as phytoplankton blooms. Such events benefit heterotrophic bacteria able to use compounds secreted by phototrophs or their biomass as major nutrient source. One example of such bacteria are Planctomycetes, which are abundant on the surfaces of marine macroscopic phototrophs; however, less data are available on their ecological roles in limnic environments. In this study, we followed a cultivation‐independent deep sequencing approach to study the bacterial community composition during a cyanobacterial bloom event in a municipal duck pond. In addition to cyanobacteria, which caused the bloom event, members of the phylum Planctomycetes were significantly enriched in the cyanobacteria‐attached fraction compared to the free‐living fraction. Separate datasets based on isolated DNA and RNA point towards considerable differences in the abundance and activity of planctomycetal families, indicating different activity peaks of these families during the cyanobacterial bloom. Motivated by the finding that the sampling location harbours untapped bacterial diversity, we included a complementary cultivation‐dependent approach and isolated and characterized three novel limnic strains belonging to the phylum Planctomycetes.

中文翻译：

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浮游植物大量繁殖期间市政鸭池中细菌群落的分析和 Anatilimnocola agregata 基因的分离。十一月，sp。十一月，Lacipirellula limnantheis sp。十一月 和 Urbifossiella limnaea gen。十一月，sp。十一月 属于浮游菌门

湖泊和池塘等水体是受人类影响的脆弱环境。合适的条件会导致光养生物的大量生长，通常称为浮游植物大量繁殖。这些事件使异养细菌受益，它们能够使用光养生物或其生物质分泌的化合物作为主要营养来源。这种细菌的一个例子是浮游菌，它们在海洋宏观光养生物的表面上很丰富。然而，关于它们在湖泊环境中的生态作用的数据较少。在这项研究中，我们采用了一种独立于培养的深度测序方法来研究市政鸭塘蓝藻水华事件期间的细菌群落组成。除了引起花华事件的蓝藻外，浮游菌门的成员与自由生活部分相比，蓝藻附着部分显着富集。基于分离的 DNA 和 RNA 的单独数据集表明浮菌科的丰度和活性存在相当大的差异，表明这些科在蓝藻大量繁殖期间具有不同的活动峰值。由于发现采样位置具有未开发的细菌多样性，我们采用了一种互补的培养依赖方法，并分离和表征了三种属于浮菌门的新型湖泊菌株。