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Neuroanatomy and neurochemistry of rat cornea: Changes with age
The Ocular Surface ( IF 6.4 ) Pub Date : 2020-12-17 , DOI: 10.1016/j.jtos.2020.11.005
Jiucheng He 1 , Thang Luong Pham 2 , Haydee E P Bazan 1
Affiliation  

Purpose

To characterize the entire rat corneal nerve architecture, the changes that occur with aging, and its sensory, sympathetic, and parasympathetic fiber distribution.

Methods

Sprague-Dawley rats (aged 1 day to 2 years old) of both sexes were euthanized, and the whole corneas were immunostained with protein gene product 9.5 (PGP9.5). The specimens were double-labeled with antibodies against calcitonin gene-related peptide (CGRP) and substance P (SP) as sensory nerve markers, vasoactive intestinal peptide (VIP) as a parasympathetic nerve marker, and neuropeptide Y (NPY) and tyrosine hydroxylase (TH) as markers of sympathetic fibers. Relative nerve density positive for each antibody was assessed by computer-assisted image analysis.

Results

Thick nerve trunks enter the cornea in the middle of the stroma and run towards the anterior stroma, subsequently dividing into smaller branches that penetrate upwards into the epithelium to form the subbasal nerve bundles. There was no significant difference in corneal innervation between sexes. CGRP and SP were the major sensory neuropeptides with 47.6% ± 3.5% and 34.9% ± 5.1%, respectively, of the total nerves. VIP was 18.4% ± 5.7%, and NPY and TH positive fibers took up 6.92% ± 2.66% and 2.92% ± 1.52%, respectively. Epithelial nerve density increased with age, reached full development at 5 weeks, and decreased at 120 weeks.

Conclusion

This study provides a complete nerve architecture and content of components of sensory, parasympathetic, and sympathetic nerves in the rat cornea. The normal innervation pattern described here will provide an essential baseline for investigators who use the rat model for assessing corneal pathologies that involve nerve alterations.



中文翻译:

大鼠角膜的神经解剖学和神经化学:随年龄的变化

目的

表征整个大鼠角膜神经结构,随年龄增长而发生的变化,及其感觉、交感和副交感神经纤维分布。

方法

对雌雄 Sprague-Dawley 大鼠 (1 天至 2 岁) 实施安乐死,并用蛋白质基因产物 9.5 (PGP9.5) 对整个角膜进行免疫染色。标本用降钙素基因相关肽(CGRP)和P物质(SP)抗体作为感觉神经标志物,血管活性肠肽(VIP)作为副交感神经标志物,神经肽Y(NPY)和酪氨酸羟化酶双标记。 TH) 作为交感神经纤维的标志物。通过计算机辅助图像分析评估每种抗体的相对神经密度阳性。

结果

粗大的神经干在基质中间进入角膜并向前基质延伸,随后分成较小的分支,向上穿透上皮形成基底下神经束。两性之间的角膜神经支配没有显着差异。CGRP 和 SP 是主要的感觉神经肽,分别占总神经的 47.6% ± 3.5% 和 34.9% ± 5.1%。VIP为18.4%±5.7%,NPY和TH阳性纤维分别占6.92%±2.66%和2.92%±1.52%。上皮神经密度随年龄增长而增加,5周时达到完全发育,120周时下降。

结论

该研究提供了大鼠角膜中感觉、副交感神经和交感神经的完整神经结构和成分的内容。此处描述的正常神经支配模式将为使用大鼠模型评估涉及神经改变的角膜病变的研究人员提供基本基线。

更新日期:2021-02-10
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