当前位置: X-MOL 学术Small Rumin Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genetic variants of the BMP2 and GDF9 genes and their associations with reproductive performance traits in Barki ewes
Small Ruminant Research ( IF 1.8 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.smallrumres.2020.106302
Adel H.M. Ibrahim

Abstract The aim of this study was to investigate variation in the bone morphogenetic protein 2 (BMP2) and growth differentiation factor 9 (GDF9) genes, and ascertain whether it was associated with reproductive traits in Barki ewes. Records for conception rate of ewe, lambing number of ewe, lambing rate of ewe, twining rate of ewe, total number of lambs born per ewe (TNLB), total weight of lambs born per ewe (TWLB), total number of lambs weaned per ewe (TNLW), total weight of lambs weaned per ewe (TWLW) and ewe rearing ability were obtained for 296 Barki ewes. Polymerase chain reaction–single strand conformational polymorphism (PCR–SSCP) analyses were used to detect variation in exon 2 of BMP2 and exon 1 of GDF9. The SSCP banding patterns for BMP2 revealed two variants (A1 and A2), which contained one nucleotide sequence difference (c.962A > T). Three SSCP banding patterns (C1, C2 and C3) were observed for GDF9 and these contained two nucleotide-sequence differences (c.25C > T and c.260 G > A). Association analysis between the variation in BMP2 and GDF9 and reproductive performance traits revealed that BMP2 genotype was associated (P

中文翻译:

巴基母羊 BMP2 和 GDF9 基因的遗传变异及其与繁殖性能特征的关联

摘要 本研究的目的是研究骨形态发生蛋白2 (BMP2) 和生长分化因子9 (GDF9) 基因的变异,并确定其是否与巴基母羊的生殖性状有关。母羊受胎率记录,母羊产羔数,母羊产羔率,母羊缠绕率,每只母羊出生羔羊总数(TNLB),每只母羊出生羔羊总重(TWLB),每只母羊断奶羔羊总数获得了 296 头 Barki 母羊的母羊 (TNLW)、每头母羊断奶羔羊的总重 (TWLW) 和母羊饲养能力。聚合酶链反应-单链构象多态性 (PCR-SSCP) 分析用于检测 BMP2 外显子 2 和 GDF9 外显子 1 的变异。BMP2 的 SSCP 条带模式揭示了两个变体(A1 和 A2),其中包含一个核苷酸序列差异(c.962A > T)。观察到 GDF9 的三个 SSCP 条带模式(C1、C2 和 C3),它们包含两个核苷酸序列差异(c.25C > T 和 c.260 G > A)。BMP2 和 GDF9 变异与繁殖性能性状之间的关联分析显示 BMP2 基因型相关(P
更新日期:2021-02-01
down
wechat
bug