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Rhizomicrobiomics of Caesalpinia bonducella, a wonder plant for PCOS treatment
Physiology and Molecular Biology of Plants ( IF 3.5 ) Pub Date : 2020-12-17 , DOI: 10.1007/s12298-020-00915-x
Sivasankari Ramadurai 1 , Usha Balasundaram 1
Affiliation  

Plant and rhizobacterial interactions contribute partly to a plant’s medicinal properties and are well studied through metagenomics. In this study, 16S rDNA, 18S rDNA, and ITS meta-sequencing were performed using the genomic DNA obtained from the rhizosphere of Caesalpinia bonducella—a medicinal shrub widely used to treat polycystic ovary syndrome (PCOS). Of the 665 Operational Taxonomic Units (OTUs) obtained from 16S rDNA sequencing, 23.9% comprised of microbes that increase the therapeutic value of plants (Bacillus, Paenibacillus), 6.4% belonged to stress and drought tolerant microbes (Pseudomonas, Rhizobium, Serratia), 8% belonged to plant-growth promoting rhizobacteria—predominantly Proteobacteria, and Firmicutes and the remaining were the microbes performing various other functions. Alpha diversity indexing by GAIA-metagenomics tool revealed the presence of a highly diverse group of microbes in the rhizosphere of C. bonducella; Chao.1 index (665), Shannon Weiner index (3.53), Simpson index (0.83) and Fisher index (106.13). The highly diverse microbes lingering around the roots of C. bonducella could possibly be due to a strong symbiotic association with the plant; root exudates nourish the microbes and the microbes in turn enrich the medicinal value of the plant.



中文翻译:

Caesalpinia bonducella 的根际微生物组学,一种治疗 PCOS 的神奇植物

植物和根际细菌的相互作用对植物的药用特性有部分贡献,并且通过宏基因组学得到了很好的研究。在这项研究中,使用从Caesalpinia bonducella根际获得的基因组 DNA 进行了 16S rDNA、18S rDNA 和 ITS 元测序,这是一种广泛用于治疗多囊卵巢综合征 (PCOS) 的药用灌木。从 16S rDNA 测序获得的 665 个操作分类单元 (OTU) 中,23.9% 由增加植物治疗价值的微生物组成(芽孢杆菌属、类芽孢杆菌属),6.4% 属于抗逆和耐旱微生物(假单胞菌属、根瘤菌属、沙雷氏菌属), 8% 属于促进植物生长的根际细菌——主要是变形杆菌厚壁菌其余的是执行各种其他功能的微生物。通过 GAIA 宏基因组学工具进行的 Alpha 多样性索引显示,在C. bonducella的根际中存在高度多样化的微生物;Chao.1 指数(665)、Shannon Weiner 指数(3.53)、Simpson 指数(0.83)和 Fisher 指数(106.13)。在C. bonducella根部周围徘徊的高度多样化的微生物可能是由于与植物的强烈共生关系;根系分泌物滋养微生物,微生物反过来又丰富了植物的药用价值。

更新日期:2020-12-17
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