当前位置: X-MOL 学术AMB Express › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Gα3 subunit Thga3 positively regulates conidiation, mycoparasitism, chitinase activity, and hydrophobicity of Trichoderma harzianum
AMB Express ( IF 3.7 ) Pub Date : 2020-12-17 , DOI: 10.1186/s13568-020-01162-9
Jie Ding , Jie Mei , Pei Huang , Ying Tian , Yao Liang , Xiliang Jiang , Mei Li

Heterotrimeric G-proteins are key elements of signal transduction pathways, which participate in regulating multiple biological processes in fungi including growth, conidiation, antagonism, and mycoparasitism. Among G protein subunits, Gα3 showed diverse regulatory functions in different fungi. In this study, we cloned a Gα3 subunit coding gene thga3 from T. harzianum Th33 that can antagonize Rhizoctonia solani and some other plant pathogenic fungi. A thga3 deletion strain Δthga3 was generated using the double-crossover homologous recombination strategy, and Rthga3 was generated by transforming thga3-expressing vector into the protoplasts of Δthga3 by the PEG/CaCl2-mediated method. The biological characteristics of wild-type Th33, Δthga3 and Rthga3 were evaluated. Compared with wild-type Th33, Δthga3 showed 15%, 94%, and 23% decrease in hyphal growth, conidia yield, and chitinase activity, respectively, and Δthga3 showed lower antagonistic and mycoparasitism abilities, while there were no significant differences between wild-type Th33 and Rthga3. The hyphal surface hydrophobicity of Δthga3 significantly decreased compared with those of the wild-type Th33 and Rthga3. qRT-PCR analysis revealed that transcript abundance of the hydrophobin gene (tha_09745) of Δthga3 decreased by 80% compared with that of wild-type Th33 and Rthga3. The results showed that thga3 positively regulates the growth, conidiation, hydrophobicity, chitinase activities, and mycoparasitism of Th33 towards R. solani. We hence deduced that the expression level of Tha_09745 is correlated to the hyphal hydrophobicity of Th33 and therefore affects the other biological characteristics of Th33. The findings of this report provide a foundation for elucidating the G-protein signal regulatory mechanisms of fungi.



中文翻译:

Gα3亚基Thga3积极调节哈茨木霉的分生孢子,支原体炎,几丁质酶活性和疏水性

异三聚体G蛋白是信号转导途径的关键元素,其参与调节真菌的多个生物学过程,包括生长,分生,拮抗和支原体寄生。在G蛋白亚基中,Gα3在不同真菌中表现出多种调节功能。在这项研究中,我们从哈茨木霉Th33中克隆了一个Gα3亚基编码基因thga3,该基因可以拮抗茄红枯菌和其他一些植物病原真菌。甲thga3缺失菌株Δ thga3使用双交换同源重组策略产生,和R thga3物通过转化产生thga3 -表达载体导入Δ的原生质体通过PEG / CaCl 2介导的方法合成thga3。评价了野生型Th33,Δthga3Rthga3的生物学特性。与野生型Th33相比,Δthga3的菌丝生长,分生孢子产量和几丁质酶活性分别降低了15%,94%和23%,并且Δthga3的拮抗和分枝杆菌杀伤能力较低,而两者之间没有显着差异。野生型Th33和R thga3。与野生型Th33和R thga3相比,Δthga3的菌丝表面疏水性显着降低。qRT-PCR分析显示Δ的疏水蛋白基因(tha_09745)的转录本丰富与野生型Th33和R thga3相比,thga3降低了80%。结果表明,thga3阳性调节Th33对茄形红斑病菌的生长,分生,疏水性,几丁质酶活性和支原体炎。因此,我们推论出Tha_09745的表达水平与Th33的菌丝疏水性相关,因此影响Th33的其他生物学特性。该报告的发现为阐明真菌的G蛋白信号调节机制提供了基础。

更新日期:2020-12-17
down
wechat
bug