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Effect of pH buffer and carbon metabolism on the yield and mechanical properties of bacterial cellulose produced by Komagataeibacter hansenii ATCC 53582.
Journal of Microbiology and Biotechnology ( IF 2.8 ) Pub Date : 2020-12-16 , DOI: 10.4014/jmb.2010.10054
Zhaofeng Li 1, 2, 3 , Si-Qian Chen 2, 3, 4 , Xiao Cao 3, 4 , Lin Li 2, 3, 4 , Jie Zhu 2, 3, 4 , Hongpeng Yu 1
Affiliation  

Bacterial cellulose (BC) is widely used in the food industry for products such as nata-de-coco. The mechanical properties including stiffness and viscoelasticity of BC hydrogel are determined by the hydrated fibril network. Generally, Komagataeibacter bacteria produce gluconic acids in a glucose medium, which may affect the pH, structure and mechanical properties of BC. In this work, the effect of pH buffer on the yields of Komagataeibacter hansenii strain ATCC 53582 was studied. The bacterium in phosphate and phthalate buffer with low ionic strength produced a good BC yield (5.16 and 4.63 g/L respectively), but there was a substantial reduction in pH due to the accumulation of gluconic acid. However, the addition of gluconic acid enhanced polymer density and mechanical properties of BC hydrogels. Its effect was similar to the bacteria using glycerol in another carbon metabolism circuit which provided good pH stability and higher conversion rate of carbon. This study broadens the understanding of how carbon sources affect BC biosynthesis.

中文翻译:

pH 缓冲液和碳代谢对 Komagataeibacter hansenii ATCC 53582 产生的细菌纤维素的产量和机械性能的影响。

细菌纤维素 (BC) 广泛用于食品工业,用于制作椰果等产品。BC 水凝胶的机械性能,包括刚度和粘弹性,由水合原纤维网络决定。通常,Komagataeibacter细菌在葡萄糖培养基中产生葡萄糖酸,这可能会影响 BC 的 pH 值、结构和机械性能。在这项工作中,pH 缓冲液对Komagataeibacter hansenii产量的影响研究了菌株 ATCC 53582。低离子强度的磷酸盐和邻苯二甲酸盐缓冲液中的细菌产生了良好的 BC 产量(分别为 5.16 和 4.63 g/L),但由于葡萄糖酸的积累,pH 值显着降低。然而,葡萄糖酸的加入提高了 BC 水凝胶的聚合物密度和机械性能。其效果类似于在另一个碳代谢回路中使用甘油的细菌,提供良好的pH稳定性和更高的碳转化率。这项研究拓宽了对碳源如何影响 BC 生物合成的理解。
更新日期:2020-12-18
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