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dsDNA-templated fluorescent copper nanoparticles for the detection of lipopolysaccharides
Analytical Methods ( IF 3.1 ) Pub Date : 2020-12-8 , DOI: 10.1039/d0ay01906b
Radhika N. K. 1, 2, 3 , Sai Siva Gorthi 1, 2, 3
Affiliation  

The introduction of lipopolysaccharides (LPS) or endotoxins that originate from Gram-negative bacteria into the human blood stream induces a severe immune response that can lead to septic shock, and even death. Hence, the accurate detection of LPS is of great importance in the medical and pharmaceutical sectors. This paper proposes a novel label-free fluorescence assay for the detection of LPS utilizing aptamers and the interference synthesis of dsDNA-templated copper nanoparticles. The assay can be performed at room temperature and does not require expensive reagents. The proposed assay has a limit of detection of 0.95 ng ml−1 of LPS, and the fluorescence emission from the copper nanoparticles was found to vary linearly with the concentration of LPS over a wide range (1 to 105 ng ml−1) with R2 = 0.9877.

中文翻译:

dsDNA模板荧光铜纳米颗粒用于脂多糖的检测

来自革兰氏阴性细菌的脂多糖(LPS)或内毒素的引入会引起严重的免疫反应,从而导致败血性休克甚至死亡。因此,LPS的准确检测在医疗和制药领域非常重要。本文提出了一种新的无标记的荧光检测方法,用于利用适体检测LPS和dsDNA模板铜纳米颗粒的干扰合成。该测定可以在室温下进行,不需要昂贵的试剂。拟议的测定法的LPS的检出限为0.95 ng ml -1,并且发现铜纳米颗粒的荧光发射随LPS的浓度在很宽的范围内(1至10 5 ng ml-1), R 2 = 0.9877。
更新日期:2020-12-16
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