Background:

Acute kidney injury (AKI) is the main clinical concern resulted from ischemia-reperfusion injury (IRI). Ample clinical data indicates that AKI is associated with distant organ dysfunctions and poor patients’ outcomes. Oxidative stress and inflammation have a critical role in the pathogenesis of organ injuries following IRI. The objectives of this study were to determine the impact of Gamma Oryzanol (GO), extracted from rice bran oil, on distant organs in rats after IRI.

Methods:

Twelve out of 24 Wistar rats were treated by one dosage of GO (100mg/kg) 1 h before I/R induction through both oral gavage and intraperitoneal injection. Then, the AKI model rats were induced by IRI. Oxidative stress and antioxidant protein levels were assessed in the brain, heart, and liver tissues in the experimental groups. Furthermore, the effects of GO on IRI-induced liver dysfunction, apoptosis, and inflammation were measured by Western blot.

Results:

GO pretreatment could significantly restore the levels and activity of antioxidant proteins in the brain, heart, and liver tissues (P < 0.05). Moreover, GO pretreatment could decrease the inflammatory cytokine (IL-1, IL-6, and TNF-α) in the liver (P < 0.01). By reducing Bax/Bcl-2 ratio and down-regulating caspase-3, GO could significantly diminish apoptosis in the liver tissue after the kidney I/R (P < 0.01). Additionally, GO could significantly diminish the deterioration of liver function in the kidney I/R model.

Conclusion:

GO protects distant organs against renal IRI-induced oxidative stress. Furthermore, it ameliorates liver function and remarkably exerts anti-oxidative, anti-inflammatory, and anti-apoptotic roles in the liver as an important detoxifying organ.

中文翻译：

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γ-谷维素对大鼠肾脏缺血再灌注后远端器官的保护作用：以肝脏保护为重点

背景：

急性肾损伤 (AKI) 是缺血再灌注损伤 (IRI) 引起的主要临床问题。大量临床数据表明 AKI 与远处器官功能障碍和患者预后不良有关。氧化应激和炎症在 IRI 后器官损伤的发病机制中起关键作用。本研究的目的是确定从米糠油中提取的谷维素 (GO) 对 IRI 后大鼠远端器官的影响。

方法：

24 只 Wistar 大鼠中有 12 只在 I/R 诱导前 1 小时通过一剂 GO (100mg/kg) 进行治疗，通过口服管饲法和腹膜内注射。然后，通过IRI诱导AKI模型大鼠。在实验组的大脑、心脏和肝脏组织中评估氧化应激和抗氧化蛋白水平。此外，通过蛋白质印迹测量 GO 对 IRI 诱导的肝功能障碍、细胞凋亡和炎症的影响。

结果：

GO预处理可显着恢复大脑、心脏和肝脏组织中抗氧化蛋白的水平和活性（P < 0.05）。此外，GO预处理可以降低肝脏中的炎性细胞因子（IL-1、IL-6和TNF-α）（P < 0.01）。通过降低 Bax/Bcl-2 比值和下调 caspase-3，GO 可显着减少肾脏 I/R 后肝组织的细胞凋亡（P < 0.01）。此外，GO 可以显着减少肾脏 I/R 模型中肝功能的恶化。

结论：

GO 保护远处器官免受肾脏 IRI 诱导的氧化应激。此外，它还可以改善肝功能，并在肝脏作为重要的解毒器官发挥显着的抗氧化、抗炎和抗凋亡作用。