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Daily oxygen supplementation to the incubator at different stages of embryonic development alters the activity of antioxidant enzymes in the lung tissue of broiler chicks at a high altitude
British Poultry Science ( IF 2 ) Pub Date : 2020-12-16
Elif Babacanoğlu, Uğur Güller

Abstract

1. Pulmonary hypertension, associated with mitochondrial function in the lung tissue of broilers, can occur at hypoxic high altitudes. The present research examined the impacts of O2 supplementation at different embryonic stages on broiler organ development and antioxidant enzyme activities.

2. In total, 360 eggs from Ross 308 broiler breeders at sea level were divided into a control group (O2 non-supplementation group) and three experimental groups daily 1 h 23.5 % O2 supplementation from days 0 to 11 (O0-11), from days 12 to 21 (O12-21), and from days 18 to 21 (O18-21) of embryonic age.

3. The lung, heart, right ventricular (RV), and left ventricular (LV) + septum of newly hatched and seven day old chicks from every group were dissected and weighed. Antioxidant enzyme activities were examined in lung tissues of them.

4. The lung weight did not change in any group. At hatching, the heart weight (g and %) was higher in the O12-21 and O18-21 groups than in the O0-11 and control groups, but the relative heart weight was the highest in the O18-21 group in comparison with the O12-21 group.

5. Superoxide dismutase (SOD) activity increased in all experimental groups at both ages, but glutathione peroxidase (GPx) activity increased only in seven day old chicks. Catalase (CAT) exhibited high activity in the O12-21 and O18-21 groups at hatching. In the seven day old chicks, while the CAT activity did not change in the O18-21 group, it decreased in the O0-11 group and increased in the O12-21 group.

7. Glutathione reductase (GR) activity did not change in the O18-21 group, but GR exhibited low activity in the O0-11 group and high activity in the O18-21 group in newly hatched chicks. The GR activity only decreased in the O18-21 group of seven day old chicks.

8. The results indicated that oxygen supplementation to the incubator caused alterations in the antioxidant enzyme activities in the lungs of broiler chicks, and this may have been in response to oxidative stress.



中文翻译:

在胚胎发育的不同阶段,每天向孵化器中补充氧气会改变高海拔地区肉鸡肺组织中抗氧化酶的活性

摘要

1.肺动脉高压与肉鸡肺组织中的线粒体功能有关,可发生在低氧高海拔地区。本研究研究了在不同胚胎阶段补充O 2对肉鸡器官发育和抗氧化酶活性的影响。

2.总共将来自罗斯308种肉鸡的海产鸡蛋360个从海平面分为对照组(O 2不补充组)和三个实验组,从第0天到第11天每天1 h 23.5%的O 2补充(O 0- 11),从胚胎年龄的12到21天(O 12-21),以及从18到21天(O 18-21)。

3.解剖并称重每组新孵化的雏鸡和7日龄小鸡的肺,心脏,右心室(RV)和左心室(LV)+隔膜。检查了它们的肺组织中的抗氧化酶活性。

4.肺重量在任何组中都没有变化。孵化时,O 12-21和O 18-21组的心脏重量(g和%)高于O 0-11和对照组,但是相对心脏重量在O 18-21中最高与O 12-21组相比。

5.在两个年龄段的所有实验组中,超氧化物歧化酶(SOD)活性均增加,但仅7日龄雏鸡中的谷胱甘肽过氧化物酶(GPx)活性增加。孵化时过氧化氢酶(CAT)在O 12-21和O 18-21组中表现出高活性。在7天大的雏鸡中,虽然O 18-21组的CAT活性没有变化,但O 0-11组的CAT活性降低,而O 12-21组的CAT活性升高。

7.谷胱甘肽还原酶(GR)的活性没有将O在改变18-21基团,但GR将O中表现出低活性0-11组和高活性将O中18-21在新孵化的小鸡组。GR活性仅在7天大的O 18-21组中降低。

8.结果表明,向培养箱中补充氧气会导致肉鸡肺部抗氧化酶活性发生变化,这可能是对氧化应激的反应。

更新日期:2020-12-16
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