ABSTRACT

The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fungus Rhizopus oligosporus in solid-state fermentation. The present study provides the first insights on production of L-carnitine-enriched edible fungal biomass through submerged cultivation of several Ascomycetes and Zygomycetes including Aspergillus oryzae, Neurospora intermedia, Rhizopus oryzae, and Rhizopus oligosporus. A. oryzae with 3 mg L-carnitine yield per gram of fungal biomass, indicates great potential on production of this bioactive compound which is remarkably higher than the other tested fungi in this work and also previous studies. In addition to fungal strain, other factors such as cultivation time and presence of yeast extract were found to play a role. Further studies on submerged growth optimization of A. oryzae in both high-quality recipes and in medium based on low-value substrates are proposed in order to clarify its potential for production of L-carnitine-enriched fungal biomass.

摘要

食用丝状真菌是未来供应功能性食品和饲料的热门候选者，其中包括蛋白质、必需氨基酸和具有免疫刺激活性的化合物。在能量代谢中起关键作用的左旋肉碱是一种功能性化合物，通常由接合菌丝状真菌Rhizopus oligosporus在固态发酵中产生。本研究提供了关于通过水下培养几种子囊菌和接合菌（包括米曲霉、中间脉孢菌、米根霉和少孢根霉）生产富含左旋肉碱的食用真菌生物质的初步见解。米曲霉每克真菌生物质产生 3 毫克左旋肉碱，表明这种生物活性化合物的生产潜力巨大，在这项工作和以前的研究中，这种生物活性化合物明显高于其他测试的真菌。除了真菌菌株外，还发现其他因素如培养时间和酵母提取物的存在也有影响。进一步研究A的潜伏生长优化。为了阐明其生产富含左旋肉碱的真菌生物质的潜力，提出了高质量配方和基于低价值底物的培养基中的米菌。