Abstract

Circular RNA (circRNA) is a new non-coding RNA with a highly conserved and stable covalently closed loop structure, and it plays an important role in a variety of biological processes and the occurrence of diseases. Based on the sequencing results, circRNA_3079 had the most significant difference between the infected group and normal group, up to about 8 times. It has attracted our attention and was selected for further verification and analysis. Though the characteristics analysis of circRNA_3079 in chicken, we found circRNA_3079 is a stable, circular transcript, which mainly exists in the cytoplasm. And it is widely expressed in various tissues of chickens, and highly expressed in lung, spleen, lymph and bursa of fabricius. Bioinformatics analysis results showed that circRNA_3079 and the predicted target genes are enriched in many pathways related to immunity or tumors, such as p53 signaling pathway, Jak−STAT signaling pathway and NOD−like receptor signaling pathway, which revealed that circRNA_3079 may indirectly regulate the ALV-J infection process through the regulation of target genes.

• HIGHLIGHTS

• CircRNA_3079 is an abundant and stable circular RNA expressed in different tissues and cells in chicken.

• The circularization of circRNA_3079 does not depend on the reverse complementary repetitive sequence of the flanking sequence.

• CircRNA_3079 may indirectly regulate the ALV-J infection process.

摘要

环状RNA（circRNA）是一种新型的非编码RNA，具有高度保守和稳定的共价闭环结构，在各种生物学过程和疾病的发生中起着重要的作用。根据测序结果，circRNA_3079在感染组和正常组之间的差异最大，约为8倍。它引起了我们的注意，并被选择进行进一步的验证和分析。通过对鸡circRNA_3079的特征分析，发现circRNA_3079是稳定的环状转录本，主要存在于细胞质中。它在鸡的各种组织中广泛表达，在肺，脾，淋巴和法氏囊中高表达。

• 强调

• CircRNA_3079是在鸡的不同组织和细胞中表达的丰富而稳定的环状RNA。

• circRNA_3079的环化不依赖于侧翼序列的反向互补重复序列。

• CircRNA_3079可间接调节ALV-J感染过程。