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Photodynamic inactivation of Staphylococcus aureus using tetraethylene glycol-substituted Zn(II) phthalocyanine
Biotechnic & Histochemistry ( IF 1.6 ) Pub Date : 2020-12-16
Ergun Mete, Nilgün Kabay, Fabienne Dumoulin, Vefa Ahsen, Sinem Tuncel Kostakoğlu, Çağrı Ergin

ABSTRACT

Methicillin resistant Staphylococcus aureus infections are increasing, especially in intensive care units. A new method for photodynamic inactivation (PDI) generates reactive oxygen species by photosensitization to kill bacteria. We investigated the PDI effect of tetraethylene glycol-substituted Zn(II) phthalocyanine (TEG-P) on S. aureus strains including two standards (ATCC 25923 and ATCC 43400) and 20 clinically isolated methicillin sensitive and 20 methicillin resistance strains. We also investigated three treated groups: 650 nm laser only, TEG-P only and TEG-P + laser, plus one control group. Treatments included 0.5, 1, 2, 4, 8, 16, 32 µg/ml concentrations of TEG-P. No suppression of bacterial growth was observed in the control, laser only and TEG-P only groups whether or not S. aureus was methicillin resistant. Bacterial growth was suppressed by 85% using 8 µg/ml TEG-P and completely suppressed by 32 µg/ml TEG-P in the TEG-P + laser group. A combination of TEG-P + laser treatment may be an alternative to conventional antibiotics for routine treatment of S. aureus infections, although further investigation of the effect at the tissue level is required.



中文翻译:

四甘醇取代的锌(II)酞菁对金黄色葡萄球菌的光动力学灭活

摘要

耐甲氧西林的金黄色葡萄球菌感染正在增加,特别是在重症监护室。一种新的光动力灭活方法(PDI)通过光敏作用产生活性氧来杀死细菌。我们调查了四甘醇取代的Zn(II)酞菁锌(TEG-P)对金黄色葡萄球菌菌株(包括两个标准品(ATCC 25923和ATCC 43400))和20个临床分离的对甲氧西林敏感的菌株和20个对甲氧西林耐药的菌株的PDI效应。我们还研究了三个治疗组:仅650 nm激光,仅TEG-P和TEG-P +激光,以及一个对照组。处理包括浓度为0.5、1、2、4、8、16、32μg/ ml的TEG-P。在对照组,仅激光组和仅TEG-P组中均未观察到细菌生长受到抑制金黄色葡萄球菌是耐甲氧西林的。在TEG-P +激光治疗组中,使用8 µg / ml TEG-P可将细菌生长抑制85%,并完全抑制32 µg / ml TEG-P。TEG-P +激光治疗的组合可能是常规抗生素治疗金黄色葡萄球菌感染的常规抗生素的替代方法,尽管还需要进一步研究组织水平的作用。

更新日期:2020-12-16
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