The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L⁻¹) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R² for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L⁻¹: 75.66 and 98.14 mg L⁻¹ d⁻¹, for T. lutea and P. tricornutum, respectively; 0.4 g L⁻¹: 129.9 and 158.47 mg L⁻¹ d⁻¹, T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L⁻¹: 1.14 and 1.41 mg L⁻¹ d⁻¹, T. lutea and P. tricornutum; 0.4 g L⁻¹: 2.09 and 1.73 mg L⁻¹ d⁻¹, T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization.

中文翻译：

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在室外条件下利用棕色微藻生产和监测生物量和岩藻黄质

在两种微藻Tisochrysis lutea和Phaeodactylum tricornutum中研究了光对生物量和岩藻黄质 (Fx) 生产力的影响。在室外中试规模的平板光生物反应器中以半连续培养模式测试了高和低生物质浓度（1.1 和 0.4 g L ^{-1 ）。}荧光光谱与化学计量学建模相结合，用于开发用于两种微藻物种的 Fx 含量和生物量浓度的预测模型。预测模型显示细胞浓度 (.93) 和 Fx 含量 (.77) 的R ²较高。对于两种微藻（1.1 g L ^-1： 75.66 和 98.14 mg L ^-1  d ^-1，分别为T. lutea和P. tricornutum；0.4 g L ^-1：129.9 和 158.47 mg L ^-1  d ^-1，T. lutea和P. tricornutum )。在 Fx 生产率中观察到相同的趋势（1.1 g L ^-1：1.14 和 1.41 mg L ^-1 d ^-1、黄斑毛霉和三角褐指藻；0.4 g L ^-1：2.09 和 1.73 mg L ^-1  d ^-1 , T. lutea和P. tricornutum）。这些结果表明，可以通过控制室外条件下的生物质浓度来设定生物质和 Fx 生产力，并且可以使用荧光光谱法进行预测。该监控工具为在线过程控制和优化开辟了新的可能性。