At present, the use of biological methods to degrade high ammonia nitrogen wastewater is a current research hotspot. In order to screen out high-efficiency ammonia-oxidizing bacteria for constructing compound bacteria and explore their performance characteristics, three high-efficiency ammonia-oxidizing bacteria which were identified as Paracoccus pantotrophus, Alcaligenes sp. and Gordonia terrae, respectively were isolated and purified from the wastewater of Maoming Petrochemical Wastewater Treatment Plant. To further improve the ammonia nitrogen degradation efficiency, the best combination in different proportions by orthogonal method. When the compound bacteria agent combination by 4:1:4 the degradation efficiency reached 47.09 mg L⁻¹ d⁻¹. The constructed compound bacterial agent was tested in Maoming Petrochemical Sewage Treatment Plant in a pilot test. Degradation experiment was carried out in a 40 L explosive gas stirring device with an initial ammonia nitrogen of 155.8 mg L⁻¹, the ammonia nitrogen was completely degraded after 48 h, and the degradation efficiency reached 77.5 mg L⁻¹ d⁻¹. The activated sludge in the pilot plant was collected and its metagenomics sequencing was performed. The results showed that the abundance of Alcaligenes aecalis and Paracoccus sp. was reached 62 % and 18 %, respectively. It is shown that the compound bactericide has high ammonia nitrogen degradation ability in the environment of simulated sewage treatment plant.

目前，利用生物方法降解高氨氮废水是当前研究的热点。为了筛选出高效率的氨氧化细菌用于构建化合物的细菌和探索它们的性能特性，这被确定为三个高效率氨氧化细菌副球菌pantotrophus，产碱杆菌属。分别从茂名石化废水处理厂的废水中分离和纯化了Gordonia和Gordonia terrae。为了进一步提高氨氮的降解效率，最好采用正交法按不同比例最佳组合。当复合细菌剂以4：1：4组合时，降解效率达到47.09 mg L ^-1 d ^-1。在茂名石化污水处理厂对构建的复合细菌剂进行了中试测试。在40 L爆炸性气体搅拌装置中，以初始氨氮为155.8 mg L ^-1进行降解实验，氨氮在48 h后完全降解，降解效率达到77.5 mg L ^-1  d ^-1。收集中试工厂中的活性污泥，并进行宏基因组测序。结果表明，丰富的产碱杆菌和副球菌sp。分别达到62％和18％。结果表明，该复合杀菌剂在模拟污水处理厂环境中具有较高的氨氮降解能力。