Abstract—The technology for producing human induced pluripotent stem cells (iPSCs) and the possibility of directed differentiation into specialized cells of all body tissues have opened unique opportunities for studying the molecular genetic basis of the pathogenesis of neurodegenerative diseases in vitro and effective screening for compounds with neuroprotective activity. The aim of this work was to obtain glial cell cultures from iPSCs of a healthy donor and a patient with the familial form of Parkinson’s disease (G2019S mutation in the LRRK2) and to characterize them. At the first stage, we compared the three previously described protocols for the differentiation of glial cells from neural precursors of human iPSCs and selected the method most acceptable under our conditions in terms of the quality and time necessary for obtaining the desired cultures. Glial cell cultures obtained by this method were characterized by the levels of expression of a number of neuroglial differentiation genes. Also, in the resulting cultures, we analyzed the expression of genes of some neurotrophic factors (GDNF, BDNF, NGF, NT3). It was shown that the culture medium conditioned by glial cells from a patient with Parkinson’s disease had a negative effect on the growth of neurites of dopaminergic neurons in differentiated cultures of a healthy donor, decreasing their length by a factor of 2.

摘要—生产人诱导多能干细胞（iPSC）的技术以及定向分化为所有人体组织的专门细胞的可能性为研究神经退行性疾病的发病机理的分子遗传学基础以及化合物的有效筛选提供了独特的机会具有神经保护活性。这项工作的目的是从健康供体和家族性帕金森氏病（LRRK2中的G2019S突变）患者的iPSC获得胶质细胞培养物。）并对其进行表征。在第一阶段，我们比较了上述三种从人iPSC的神经前体分化神经胶质细胞的方案，并根据获得所需培养物所需的质量和时间选择了在我们的条件下最可接受的方法。通过这种方法获得的神经胶质细胞培养物的特征在于许多神经胶质分化基因的表达水平。此外，在所得的培养物中，我们分析了一些神经营养因子（GDNF，BDNF，NGF，NT3）。结果表明，以帕金森氏病患者的神经胶质细胞为条件的培养基对健康捐献者分化培养物中多巴胺能神经元神经突的生长具有负面影响，其长度减少了2倍。