Significance: Processing and diagnosing a set of 12 prostate biopsies using conventional histology methods typically take at least one day. A rapid and accurate process performed while the patient is still on-site could significantly improve the patient’s quality of life. Aim: We develop and assess the feasibility of a one-hour-to-diagnosis (1Hr2Dx) method for processing and providing a preliminary diagnosis of a set of 12 prostate biopsies. Approach: We developed a fluorescence staining, optical clearing, and 3D open-top light-sheet microscopy workflow to enable 12 prostate needle core biopsies to be processed and diagnosed within an hour of receipt. We analyzed 44 biopsies by the 1Hr2Dx method, which does not consume tissue. The biopsies were then processed for routine, slide-based 2D histology. Three pathologists independently evaluated the 3D 1Hr2Dx and 2D slide-based datasets in a blinded, randomized fashion. Turnaround times were recorded, and the accuracy of our method was compared with gold-standard slide-based histology. Results: The average turnaround time for tissue processing, imaging, and diagnosis was 44.5 min. The sensitivity and specificity of 1Hr2Dx in diagnosing cancer were both >90 % . Conclusions: The 1Hr2Dx method has the potential to improve patient care by providing an accurate preliminary diagnosis within an hour of biopsy.

中文翻译：

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通过开顶光片显微镜在活检后一小时内诊断 12 个前列腺针芯

意义：使用常规组织学方法处理和诊断一组 12 个前列腺活检通常至少需要一天时间。在患者仍在现场的情况下执行快速准确的过程可以显着改善患者的生活质量。目的：我们开发并评估了一种用于处理和提供一组 12 个前列腺活检的初步诊断的一小时诊断 (1Hr2Dx) 方法的可行性。方法：我们开发了荧光染色、光学透明和 3D 开顶光片显微镜工作流程，使 12 个前列腺针芯活检能够在收到后一小时内得到处理和诊断。我们通过不消耗组织的 1Hr2Dx 方法分析了 44 个活检组织。然后对活组织检查进行常规的、基于幻灯片的 2D 组织学处理。三位病理学家以盲法、随机方式独立评估 3D 1Hr2Dx 和 2D 幻灯片数据集。记录周转时间，并将我们方法的准确性与基于载玻片的金标准组织学进行比较。结果：组织处理、成像和诊断的平均周转时间为 44.5 分钟。1Hr2Dx诊断癌症的敏感性和特异性均>90%。结论：1Hr2Dx 方法有可能通过在活检后一小时内提供准确的初步诊断来改善患者护理。1Hr2Dx诊断癌症的敏感性和特异性均>90%。结论：1Hr2Dx 方法有可能通过在活检后一小时内提供准确的初步诊断来改善患者护理。1Hr2Dx诊断癌症的敏感性和特异性均>90%。结论：1Hr2Dx 方法有可能通过在活检后一小时内提供准确的初步诊断来改善患者护理。