Abstract

Purpose

This study aimed to investigate the impact of gamma radiation of Musca domestica males (resulted from irradiated pupae) crossed with unirradiated females on fecundity, egg hatchability, adult emergence, sex ratio, sterility, in addition to reproductive development at the level of oogenesis and spermatogenesis compared to unirradiated group.

Material and methods

The housefly, M. domestica pupae were exposed to three sublethal doses of 5, 10, and 15 Gy.

Results

Fecundity was severely reduced particularly in F2 (11.33 ± 1.528; 7.33 ± 1.115 eggs/♀) and F3 (9.0 ± 1.00; 4.67 ± 1.115 eggs/♀) for doses of 10 and 15 Gy, respectively, compared with (52.0 ± 1.4 eggs/♀) for the control. Data revealed latent dose- and generation-dependent reduction in egg hatchability. Hatchability percentages reduced from 93.59 for the control to 10.07 (F1), 8.09 (F2), and 8.34 (F3) when the highest radiation dose 15 Gy was applied. Irradiation induced paternal deleterious substerility effects. Irradiation with 15 Gy induced substerility that reached about 97.0% in F2 and F3 generations. A significant (P < 0.05) reduction of the mean numbers of adult emergence was remarkably detected in the F1, F2, and F3 generations. Applied gamma doses did not affect the male to female ratio in the Parental or F1 generations. However, the F2 and F3 generations did show changes to the sex ratio with males occurring more frequently than females. This trend became more pronounced as dose increased. Ultrastructural examinations exhibited unusual damage and malformation either for males or female reproductive organs.

Conclusion

The obtained results clearly show that gamma radiation of M. domestica irradiated as pupae induced considerably visible impact on tested biological aspects and reproductive potential.

中文翻译：

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γ 辐射的父本传播对家蝇繁殖、卵子发生和精子发生的影响，家蝇，家蝇（双翅目：蝇科）

摘要

目的

本研究旨在调查家蝇雄性（由受辐射的蛹产生）与未受辐射的雌性杂交的伽马辐射对繁殖力、卵孵化率、成虫出现、性别比、不育以及在卵子发生和精子发生水平上的生殖发育的影响与未照射组相比。

材料与方法

家蝇，家蝇蛹暴露至三个亚致死剂量5，10，和15戈瑞。

结果

与 (52.0 ± 1) 剂量分别相比，F2 (11.33 ± 1.528; 7.33 ± 1.115 个鸡蛋/♀) 和 F3 (9.0 ± 1.00; 4.67 ± 1.115 个鸡蛋/♀) 的繁殖力显着降低。 /♀) 为控制。数据显示鸡蛋孵化率的潜在剂量和代依赖性降低。当应用最高辐射剂量 15 Gy 时，孵化率百分比从对照的 93.59 降至 10.07 (F1)、8.09 (F2) 和 8.34 (F3)。辐照诱导父本有害的亚不育效应。15 Gy 辐照诱导的不育率在 F2 和 F3 代中达到约 97.0%。显着 ( P < 0.05) 在 F1、F2 和 F3 代中显着地检测到成虫羽化平均数的减少。施加的伽马剂量不影响亲代或 F1 代的男女比例。然而，F2 和 F3 代确实显示了性别比例的变化，男性比女性出现的频率更高。随着剂量的增加，这种趋势变得更加明显。超微结构检查显示男性或女性生殖器官出现异常损伤和畸形。

结论

将所得到的结果清楚地显示的该γ辐射家蝇照射作为测试生物方面和繁殖潜力蛹诱导相当明显的影响。