The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.

胰腺β细胞产生的肽激素胰岛素在分泌前经过转录后加工。特别地，将C-肽从胰岛素原切割下来以产生成熟的胰岛素。在这里，我们介绍一个C肽mCherry人iPSC系（HMGUi001-A-8）。该品系是由CRISPR / Cas9介导的mCherry序列杂合插入胰岛素基因座第3外显子而产生的。我们证明该线是多能的，并有效地分化为胰腺β样细胞，该细胞定位于包含胰岛素的颗粒中的红色荧光C肽-mCherry融合蛋白。因此，HMGUi001-A-8系是宝贵的资源，可纯化衍生的β样细胞并实时跟踪含胰岛素的颗粒。