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Endogenous auxin accumulation/localization during zygotic and somatic embryogenesis of Capsicum chinense Jacq
Journal of Plant Physiology ( IF 4.3 ) Pub Date : 2021-03-01 , DOI: 10.1016/j.jplph.2020.153333
Jacobo Pérez-Pastrana , Pilar S. Testillano , Ivett Barany , Adriana Canto-Flick , Dulce Álvarez-López , Gema Pijeira-Fernández , Susana A. Avilés-Viñas , Laura Peña-Yam , Liliana Muñoz-Ramírez , Sara Nahuat-Dzib , Ignacio Islas-Flores , Nancy Santana-Buzzy

Abstract Zygotic and somatic embryogenesis in plants is a fascinating event that is finely regulated through the expression of a specific group of genes and dynamic levels of plant hormones whose concerted action determines the fate that specific cells follow towards zygotic or somatic embryo development. This work studied different stages of Capsicum chinense Jacq. zygotic and somatic embryogenesis. HPLC quantification determined that the levels of indole-3-acetic acid (IAA) increase as the zygotic or somatic embryogenesis progresses, being higher at maturity, thus supporting a positive correlation between embryo cell differentiation and IAA increase. A monoclonal anti-IAA-antibody was used to detect IAA levels. Findings revealed a dynamic pattern of auxin distribution along the different embryogenic embryonic stages. In the early stages of zygotic embryos, the IAA gradient was observed in the basal cells of the suspensor and the hypostases, suggesting that they are the initial source of the IAA hormone. As embryogenesis proceeds, the dynamic of the IAA gradient is displaced to the embryo and endosperm cells. In the case of induced somatic embryogenesis, the IAA gradient was detected in the dividing cells of the endodermis, from where pre-embryogenic cells emerge. However, the analysis of somatic embryos revealed that IAA was homogeneously distributed. This study shows evidence supporting a correlation between IAA levels during zygotic or somatic embryogenesis in Capsicum chinense species.

中文翻译:

辣椒合子和体细胞胚胎发生过程中内源生长素的积累/定位

摘要 植物中的合子和体细胞胚胎发生是一个引人入胜的事件,通过特定基因组的表达和植物激素的动态水平进行精细调节,这些激素的协同作用决定了特定细胞遵循合子或体细胞胚胎发育的命运。这项工作研究了辣椒的不同阶段。合子和体细胞胚胎发生。HPLC 定量确定吲哚-3-乙酸 (IAA) 的水平随着合子或体细胞胚胎发生的进展而增加,在成熟时更高,因此支持胚胎细胞分化和 IAA 增加之间的正相关。使用单克隆抗 IAA 抗体检测 IAA 水平。研究结果揭示了生长素在不同胚胎发生阶段的动态分布模式。在合子胚胎的早期阶段,在悬质和胚体的基底细胞中观察到 IAA 梯度,表明它们是 IAA 激素的初始来源。随着胚胎发生的进行,IAA 梯度的动态被转移到胚胎和胚乳细胞。在诱导体细胞胚胎发生的情况下,在内胚层的分裂细胞中检测到 IAA 梯度,从那里出现前胚细胞。然而,体细胞胚胎的分析表明 IAA 分布均匀。该研究显示了支持在辣椒种的合子或体细胞胚胎发生过程中 IAA 水平之间存在相关性的证据。随着胚胎发生的进行,IAA 梯度的动态被转移到胚胎和胚乳细胞。在诱导体细胞胚胎发生的情况下,在内胚层的分裂细胞中检测到 IAA 梯度,从那里出现前胚细胞。然而,体细胞胚胎的分析表明 IAA 分布均匀。该研究显示了支持在辣椒种的合子或体细胞胚胎发生过程中 IAA 水平之间存在相关性的证据。随着胚胎发生的进行,IAA 梯度的动态被转移到胚胎和胚乳细胞。在诱导体细胞胚胎发生的情况下,在内胚层的分裂细胞中检测到 IAA 梯度,从那里出现前胚细胞。然而,体细胞胚胎的分析表明 IAA 分布均匀。该研究显示了支持在辣椒种的合子或体细胞胚胎发生过程中 IAA 水平之间存在相关性的证据。
更新日期:2021-03-01
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