In this work, we report the application of Raman microspectroscopy for analysis of the refractive index of a range of tissue phantoms. Using both a custom-developed setup with visible laser source and a commercial micro-spectrometer with near infrared laser, we measured the Raman spectra of gelatin hydrogels at various concentrations. By building a calibration curve from measured refractometry data and Raman scattering intensity for different vibrational modes of the hydrogel, we were able to predict the refractive indices of the gels from their Raman spectra. This work highlights the importance of a correlative approach through Brillouin-Raman microspectroscopy for the mechano-chemical analysis of biologically relevant samples.

中文翻译：

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EXPRESS：使用光散射光谱监测组织模型的折射率

在这项工作中，我们报告了拉曼显微光谱在一系列组织体模的折射率分析中的应用。使用带有可见激光源的定制开发装置和带有近红外激光的商用微型光谱仪，我们测量了各种浓度的明胶水凝胶的拉曼光谱。通过根据测量的折射数据和水凝胶不同振动模式的拉曼散射强度构建校准曲线，我们能够从拉曼光谱预测凝胶的折射率。这项工作强调了通过布里渊-拉曼显微光谱法对生物相关样品进行机械化学分析的相关方法的重要性。