The availability of reference genomes has revolutionized the study of biology. Multiple competing technologies have been developed to improve the quality and robustness of genome assemblies during the past decade. The 2 widely used long-read sequencing providers—Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)—have recently updated their platforms: PacBio enables high-throughput HiFi reads with base-level resolution of >99%, and ONT generated reads as long as 2 Mb. We applied the 2 up-to-date platforms to a single rice individual and then compared the 2 assemblies to investigate the advantages and limitations of each.

中文翻译：

---

两种最新的基因组组装测序技术比较：Pacific Biosciences Sequel II 系统的 HiFi reads 和 Oxford Nanopore 的 Ultralong reads

参考基因组的可用性彻底改变了生物学研究。在过去的十年中，已经开发了多种竞争技术来提高基因组组装的质量和稳健性。两家广泛使用的长读长测序供应商——Pacific Biosciences (PacBio) 和 Oxford Nanopore Technologies (ONT)——最近更新了他们的平台：PacBio 支持高通量 HiFi 读长，基础分辨率 >99%，ONT 生成读长只要 2 Mb。我们将 2 个最新平台应用于单个水稻个体，然后比较这 2 个组件以研究每个组件的优点和局限性。