ABSTRACT

Life adapts to various environments, including high temperatures and high pressures. The brine shrimp Artemia was used to investigate the tolerance to hydrostatic pressure up to 750 bar. The swimming activity of Artemia nauplii (larval form) decreased as pressure increased, and the activity became null at 400 bar and above. Interestingly, at 300 bar and less, the swimming activity gradually recovered even under pressure within a short period of time. Up to 500 bar, the activity was reversibly recovered by reducing the pressure to 1 bar. These results could be explained by reversible responses of protein functions and membrane structures, as well as temporal adaptation of cell functions to pressure. The upper limit pressure at which the swimming activity was reversible or irreversible matched that for macromolecular synthesis (500 bar). Altogether, these results indicate that eukaryotes and prokaryotes can temporarily adapt to a high pressure of 500 bar and less.

抽象的

生活适应各种环境，包括高温和高压。盐水虾Artemia用于研究对高达750 bar的静水压力的耐受性。卤虫的游泳活动无节幼体（幼体形式）随着压力的增加而降低，并且在400 bar及更高的压力下其活性变为零。有趣的是，在300巴及以下的压力下，即使在短时间内受到压力，游泳活动也逐渐恢复。高达500 bar，通过将压力降低至1 bar可逆地恢复活性。这些结果可以通过蛋白质功能和膜结构的可逆反应以及细胞功能对压力的时间适应性来解释。游泳活动可逆或不可逆的上限压力与大分子合成的上限压力（500 bar）匹配。总而言之，这些结果表明真核生物和原核生物可以暂时适应500 bar以下的高压。