In this study, a novel purification method using magnetic solid‐phase extraction (MSPE) based on magnetic carbon nanotube dummy molecularly imprinted polymer (MCNTMIP) nanocomposite was investigated for separation and enrichment of sulfonamide antibiotics (SAs) in fish and shrimp samples. The MCNTMIP nanocomposite was successfully synthesized by applying carbon nanotubes as supporting template, methacrylic acid as functional monomer, sulfabenzamide as the dummy template for SAs, and ethylene glycol dimethacrylate as crosslinking agent, then was characterized by Fourier‐transform infrared spectrometry and vibrating sample magnetometry. The adsorption performance of MCNTMIP was evaluated by binding experiments, including static adsorption, kinetic adsorption, and selectivity recognition study. The results confirmed that an imprinted polymer layer was successfully constructed on the surface of the MCNTMIP and this sorbent has advantages of simple magnetic separation, specific molecular recognition, and high adsorption capacity. Combined with ultra‐high performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS), we developed a rapid, sensitive, efficient MSPE method for detecting SAs analytes. Under the optimal conditions, the limits of detection were low to 0.1 μg/kg, and the recoveries of SAs analytes were ranged between 90.2 and 99.9%. In addition, the precision values were ranged between 0.5 and 9.1%. This method was successfully applied to analyze SAs in fish and shrimp samples with satisfactory recoveries.

中文翻译：

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基于磁性碳纳米管模拟分子印迹聚合物萃取-UPLC-MS/MS测定鱼虾样品中磺胺类抗生素

在本研究中，研究了一种基于磁性碳纳米管虚拟分子印迹聚合物 (MCNTMIP) 纳米复合材料的磁性固相萃取 (MSPE) 纯化方法，用于分离和富集鱼虾样品中的磺胺类抗生素 (SA)。以碳纳米管为支撑模板，甲基丙烯酸为功能单体，磺胺苯甲酰胺为SAs的虚拟模板，乙二醇二甲基丙烯酸酯为交联剂，成功合成了MCNTMIP纳米复合材料，然后通过傅里叶变换红外光谱和振动样品磁强计进行表征。MCNTMIP的吸附性能通过结合实验进行评价，包括静态吸附、动力学吸附和选择性识别研究。结果证实，在 MCNTMIP 表面成功构建了印迹聚合物层，该吸附剂具有简单的磁分离、特异性分子识别和高吸附容量的优点。结合超高效液相色谱串联质谱 (UPLC-MS/MS)，我们开发了一种快速、灵敏、高效的 MSPE 方法来检测 SA 分析物。在最佳条件下，检出限低至 0.1 μg/kg，SAs 分析物的回收率介于 90.2% 和 99.9% 之间。此外，精度值介于 0.5% 和 9.1% 之间。该方法已成功应用于分析鱼虾样品中的 SA，回收率令人满意。